Abstract

Peroxisome proliferators-activated receptor gamma (PPARG) is an important regulator in the regulation of adipose differentiation and development. The mutations of the PPARG in human had been shown to be associated with type II diabetes, fat distribution and body weight. The functional importance of the PPARG makes it a good candidate to search molecular markers in marker assistant selection in cattle breeding. All the mRNA region of the PPARG gene within 760 individuals of four Chinese cattle breeds was scanned and four single nucleotide polymorphisms (SNPs) (-110G>C, -27C>T, +20A>G, +1344G>T) of the PPARG were detected in three Chinese indigenous cattle breeds (Qinchuan, Nangyang and Jiaxian cattle), rather than Chinese Holstein cattle. The mutations -110G>C, -27C>T and +20A>G located in the Exon1 of the PPARG and were under linkage disequilibrium. The individuals with these three mutations had smaller body measurements. This information could help animal scientists to develop genetic markers or biomarkers to assist with beef production.

Key words: Peroxisome proliferators-activated receptor gamma (PPARG) gene, polymorphisms, cattle, association analysis.

Abbreviation

PPARG, Peroxisome proliferators-activated receptor gamma;MAS, Marker assistant selection; NY, Nanyang; QC, Qinchuan; JX, Jiaxian; CH,Chinese Holstein; PCR-SSCP, polymerase chain reaction single-strand conformation polymorphism; SNPs, single nucleotide polymorphisms; RFLP,restriction fragment length polymorphism; LD, Linkage disequilibrium; BMI, body mass index.