Abstract

T-Lymphocytes (T-cells) play a dominant role in the initiation and maintenance of autoimmune inflammatory processes. They become activated by the presentation of antigen such as auto antigen. A main feature of T-cell activation is the production of cytokines. Though, uncontrolled T-cells activation through T-cell receptor (TCR) has been linked to numerous autoinflammatory and autoimmune pathologies. This study investigates the impact of methanol extracts from Rosa damascena on suppress T-cells activation. Peripheral blood mononuclear cells (PBMC) were treated by 10, 50,100 μg/ml of R. damascene methanolic extracts after activation of T cells with anti- CD3 and anti-CD28. The inhibitory role of methanol extracts of R. damascena to suppress the activated T-cells was measured using flow cytometric at three different time points (24, 48, and 72 h). The result showed that the methanol extraction from R. damascena petal parts can inhibit the activated T-cells in a dose dependent fashion as we observe reduced levels of cluster of differentiation of CD 3. It is concluded that, extracts of R. damascena could be used as a new agent (immunoregulatory) to suppress T-cell activity and treating variety of immune disorders with a low risk of side effects in future.

Key words: T-lymphocytes (T-cells), autoimmune inflammatory, Rosa damascena, (CD) 3.