Full Length Research Paper
Abstract
The objectives of this study were to compare the application and utility of sequence-related ampliï¬ed polymorphism (SRAP) and target region ampliï¬cation polymorphism (TRAP) techniques for the analysis of genetic diversity among durum wheat genotypes under heat stress and to compare genetic diversity estimated using molecular markers with morpho-agronomic performance under heat stress. Six durum wheat genotypes were used in this study. They were evaluated phenotypically for heat tolerance. The dendrogram generated from standardized morpho-agronomic data separated the six durum wheat genotypes into three main groups. The dendrogram generated from the standardized morpho-agronomic data separated the six durum wheat genotypes into three clusters, which diverged at similarity index of 0.72. The dendrogram based on SRAP markers differed from thatbased on TRAP markers. The combined dendrogram (SRAP, TRAP and morpho-agronomic data) agrees better with the grouping of these durum wheat genotypes depending on pedigree and the dendrogram generated by morpho-agronomic data alone.
Key words: Durum wheat, genetic diversity, heat tolerance, morpho-agronomic, sequence-related ampliï¬ed polymorphism (SRAP) markers, target region ampliï¬cation polymorphism (TRAP) markers.
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