African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Study on transformation of cowpea trypsin inhibitor gene into cauliflower (Brassica oleracea L. var. botrytis)

LV Lingling1*   Lei Jianjun2   Song Ming3    Li Liyun4   Cao Bihao5
  1Southern Subtropical Crop Research Institute,  Zhan Jiang, Guang Dong , 524091; 2South China Agricultural University, Guang Zhou, Guang Dong , 510642; 3Southwest Agricultural University, Chong Qing, 400716; 4Agriculture & Industry & Commerce Secondary professional School, Zhan Jiang, Guang Dong , 524091 )
Email: [email protected]

  •  Accepted: 26 August 2004
  •  Published: 31 January 2005

Abstract

 

Cowpea Trypsin Inhibitor (CpTI) gene was transferred into cauliflower byagrobacterium-mediated transformation method, and 14 transgenic cauliflower plants were obtained. Cotyledons and hypocotyls were used as explants. The putative transformants were assayed by PCR and Southern blotting analysis. The results indicated that CpTI gene was transferred into cauliflower successfully. The result of preliminary insect-resistant assay showed that the transgenic plants were more resistant to Pieris rapae than non-transgenic plants.

 

Key words: cauliflower (Brassica oleracea L. var. botrytis)   agrobacterium -mediated   genetic transformation     CpTI gene    transgenic plant.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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