African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Full Length Research Paper

Development and validation of analytical methodology for quantification of total flavonoids of Morus nigra by ultraviolet-visible absorption spectrophotometry

Pedrita Alves Sampaio
  • Pedrita Alves Sampaio
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Hyany Andreysa Pereira Teixeira
  • Hyany Andreysa Pereira Teixeira
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Nathalia Andrezza Carvalho de Souza
  • Nathalia Andrezza Carvalho de Souza
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Jose Marcos Teixeira de Alencar-Filho
  • Jose Marcos Teixeira de Alencar-Filho
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Grasielly Rocha Souza
  • Grasielly Rocha Souza
  • Universidade Federal do Piauí, 64049-550, Teresina, Piauí, Brazil.
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Emanuella Chiara Valença Pereira
  • Emanuella Chiara Valença Pereira
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Raimundo Gonçalves de Oliveira-Junior
  • Raimundo Gonçalves de Oliveira-Junior
  • Núcleo de Estudos e Pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Pedro Jose Rolim-Neto
  • Pedro Jose Rolim-Neto
  • Laboratório de Tecnologia dos Medicamentos (LTM), Universidade Federal de Pernambuco, 50.670-901, Recife, Pernambuco, Brazil.
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Jackson Roberto Guedes da Silva Almeida
  • Jackson Roberto Guedes da Silva Almeida
  • Núcleo de Estudos e Pesquisas de Plantas Medicinais (NEPLAME), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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Larissa Araujo Rolim
  • Larissa Araujo Rolim
  • Central de Análises de Fármacos, Medicamentos e Alimentos (CAFMA), Universidade Federal do Vale do São Francisco, 56.304-205, Petrolina, Pernambuco, Brazil.
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  •  Received: 18 March 2018
  •  Accepted: 04 June 2018
  •  Published: 06 June 2018

Abstract

Morus nigra L. is known in the region of Brazilian São Francisco Valley as "amora-miúra". It is widely used in traditional medicine, mainly in treatment of diabetes, hypercholesterolemia, cardiovascular problems, obesity and gout. This study proposed to develop and validate a fast, simple, efficient and low cost analytical method that could quantify total flavonoid content present in crude ethanolic extract of leaves of M. nigra (Mn EtOH). For this, ultraviolet-visible (UV-Vis) absorption spectrophotometry was used as the analytical tool and the complexation with aluminium chloride (AlCl3). The quantification of flavonoids by this method was based on the complexation of AlCl3 with the flavonoid nucleus as a selectivity tool made by two procedures: without complexation (NCP) and with complexation (WCP), in order to evaluate the effects of the complexing agent in quantification, proving the efficiency of this technique. In this way, it became necessary to validate the method used to ensure its efficiency. The validation of the method of quantification of total flavonoid content by UV-Vis demonstrated that the method was selective, linear, precise, accurate, and robust.

Key words: Morus nigra, UV-Vis, Moraceae, total flavonoids, natural products.

 

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This article is published under the terms of the Creative Commons Attribution License 4.0

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