African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12495

Full Length Research Paper

Improvement of Basmati rice (Oryza sativa L.) using traditional breeding technology supplemented with molecular markers

D. Singh*, A. Kumar, A. Sirohi4, P. Kumar1, J. Singh2, V. Kumar, A. Jindal, S. Kumar, N. Kumar, V. Kumar3, V. Sharma, S. Gupta4 and S. Chand
  Molecular Biology Laboratory, Department of Genetics and Plant Breeding, 1Department of Biochemistry & Physiology, 2Department of Immunology& Defense Mechanism, 3Department of Ag. Biotechnology, AAU, Gujrat, India (Present address) 4Department of Molecular Biology & Genetic Engineering, Sardar Vallabhbhai Patel University of Agriculture and Technology, Meerut-250 110, UP (India).
Email: [email protected]

  •  Accepted: 17 September 2010
  •  Published: 24 January 2011

Abstract

 

The present study was undertaken to combine dwarfism (sd-1) from Pusa-1121 and aroma and resistance against diseases from Khalsa-7 into basmati background (Type-3). The dwarf segregants of the cross (Khalsa 7 X Pusa 1121) with Type-3 were screened under artificially created epiphytotic conditions in order to select plants free from common diseases of rice. Data recorded for yield and physical standards of quality acceptable in the global trade measured in All India Coordinated Trial Research Project (AICRP) trials were used for demonstration. In addition, two separate polymerase chain reaction (PCR) studies were conducted for detection of resistance against blast. PCR products using random amplified polymorphic DNA (RAPD) and sequence characterized amplified regions (SCAR) markers detected resistance against blast in the genotype Vallabh Basmati-21. The Basmati type, disease resistant and promising selections were assessed for lineage with traditional varieties of basmati rice. The resultant genotype VB-21 expressed close molecular lineage with traditional varieties of basmati rice as well. The present study indicated substantial utility of molecular markers to supplement traditional methods of breeding for improvement of basmati rice.

 

Key words: Basmati rice, random amplified polymorphic DNA, sequence characterized amplified regions, molecular lineage, Vallabh Basmati- 21.

Abbreviation

Abbreviations: ASV, Alkali spreading value; BLB, bacterial leaf blight; CTAB,cetyltrimethyleammoniumbromide; DNA, deoxyribose nucleic acid; ER, elongation ratio; PCR, polymerase chain reaction; RAPD, random amplified polymorphic DNA;SCAR,     sequence characterized amplified regions.