African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Histochemical GUS expression of beta tubulin promoter in transgenic tobacco

Hira Mubeen
  • Hira Mubeen
  • National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
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Aftab Bashir
  • Aftab Bashir
  • National Institute for Biotechnology and Genetic Engineering, Faisalabad, Pakistan.
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Ayesha Ameen
  • Ayesha Ameen
  • Department of Biological Sciences, Forman Christian College, Lahore, Pakistan.
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Ammara Masood
  • Ammara Masood
  • Department of Biological Sciences, Forman Christian College, Lahore, Pakistan.
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Shahid Raza
  • Shahid Raza
  • Department of Biological Sciences, Lahore Garrison University, Islamabad, Pakistan.
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  •  Received: 24 August 2016
  •  Accepted: 14 March 2017
  •  Published: 26 April 2017

Abstract

Agrobacterium mediated plant transformation is a useful technique for stable transformation of plants. This study aimed at stable transformation of the beta tubulin (β) gene promoter and its expression analysis by histochemical GUS assay. The beta tubulin promoter sequence was cloned in plant expression vector (pGA482) and transformed stably in tobacco through Agrobacterium mediated transformation. The stable GUS expression assays for this promoter in various tissues of Nicotiana tabacum indicated its functional importance in regulating gene expression in a constitutive manner. It was concluded that the β tubulin promoter is constitutively expressed with a strength equivalent to CaMV 2X35S promoter. The isolated promoter can be used in plant genetic engineering for crop improvement in future.

 

Key words: Constitutive, gene expression, GUS, transformation.

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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