African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

The effects of nitrogen deficiencies on the lipid and protein contents of Spirulina platensis

Leyla Uslu1, Oya IÅŸik1*, Kemal Koç2 and Tolga Göksan3
  1Faculty of Fisheries, Çukurova University, Adana, Turkey. 2Faculty of Education, Baskent University, Ankara, Turkey. 3Faculty of Fisheries,Çanakkale Eighteen March University, Çanakkale, Turkey.
Email: [email protected]

  •  Accepted: 23 December 2010
  •  Published: 17 January 2011

Abstract

 

Nitrogen deficiencies were studied in Spirulina platensis (Cyanophyceae) with the aim of determining the effects of the 50 and 100% deficient nitrogen on the lipid and protein contents of the cell under laboratory conditions. S. platensis cultures were  grown in Spirulina medium and kept at  the constant room temperature of 26 ± 2°C, illuminated with fluorescent lamps at an irradiance level of 80 µmol m-2 s-1 with photoperiod 16:8 (L:D) and aerated continuously. In the Spirulina biomass harvested at the stationary phase, 67.4, 53.5, 5.6% protein and 5.78, 13.66, 17.05% lipid were recorded for the groups of control, 50% N(-) and 100% N(-), respectively.The highest lipid content and 1.00 gL-1 dry-weight were recorded from the culture to which treated 100% N(-).

 

Key words: Spirulina platensis, lipid, nitrogen deficiencies, protein.