African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Optimization of a plant regeneration protocol for broccoli

Ke. Huang1,2,3*,#, Qiuyun Wu1,2#, Juncheng Lin3 and Jingui Zheng3
1Hunan provincial key laboratory for germplasm innovation and utilization of crop, Changsha, 410128, P. R. China. 2College of Horticulture and Landscape, Hunan Agricultural University, Changsha, 410128, P. R. China. 3Institute of Agricultural Product Quality, Fujian Agricultural and Forestry University, Fuzhou, 350002, P. R. China.
Email: [email protected]

  •  Accepted: 18 March 2011
  •  Published: 16 May 2011

Abstract

The factors which influence the regeneration of broccoli (Brassica oleracea var. italica) were studied using an orthogonal design. The results showed that the major factor was the explant type, followed by naphthylacetic acid (NAA), benzylaminopurine (BAP), sucrose and AgNO3 in turn. The maximum regeneration was on Murashige and Skoog (MS) medium + NAA 0.107 µM + BAP 17.76 µM + 2% sucrose + 0.8% agar. Hypocotyl proved to be the optimum explant source and its regeneration frequency reached 100%.

 

Key words: Naphthylacetic acid (NAA), benzylaminopurine (BAP), AgNO3, hypocotyl, broccoli (Brassica oleracea var. italica)

Abbreviation

Abbreviations: MS, Murashige and Skoog; BAP, benzylaminopurine; NAA,naphthylacetic acid.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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