Full Length Research Paper
Abstract
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Success in genetic engineering of cereals depends on the callus formation and efficient plant regeneration system. Callus formation and plant regeneration of wheat mature embryos were assessed by using 12 different methods with 4 genotypes. Genotype significantly affected the formation of callus, embryogenic callus and plant regeneration. Only two methods (methods #2 and 9) produced plant regeneration. The highest plant regeneration for all genotypes with endosperm that supported mature embryos was observed in method #9 which contain MS medium supplemented with 12 mg/l dicamba + 0.5 mg/l IAA.
Key words: Callus formation, explant, genotype, plant growth regulators. |
Abbreviation
MS, Murashige and Skoog (1962) medium; B5, Gamborg et al. (1968) medium; 2,4-D, 2,4-dichlorophenoxy acetic acit; Dicamba, 3,6-dichloro-2-methoxybenzoic acid; Picloram, 4-amino-3,5,6-trichloropicolinic acid; IAA, ındole-3-acetic acid; NAA, 1-naphthaleneacetic acid; BAP, 6-benzylaminopurine; TDZ,thidiazuron, 1-phenyl-3-[1,2,3 thiadozol-5-yl] urea.
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