African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Growth pattern and structural nature of amylases produced by some Bacillus species in starchy substrates

Ajayi A.O1* and Fagade O.E2
1Department of Microbiology, Adekunle Ajasin University, P.M.B 001, Akungba-Akoko, Ondo State, Nigeria. 2Department of Botany and Microbiology, University of Ibadan, Ibadan, Oyo State, Nigeria.
Email: [email protected]

  •  Accepted: 06 January 2006
  •  Published: 01 March 2006

Abstract

The growth pattern and microbial biomass formed during metabolic activities of theBacillus species on starchy substrates was determined. The result showed that the strains B. subtilis (WBS), B. licheniformis (WBL) and B. coagulans (MBC) generally had high growth rate. B. circulans (SBC) and B. coagulans (WBC) has specific affinity for growth and some enzymatic activity on corn starch medium compared with other lower growth observed in B. polymyxa (WBP) but higher enzyme production. The amylolytic Bacillus species obtained utilized white corn starch substrate as a sole carbon source as well as soluble starch. The amylase production values range from 0.022 x 102 unit/cfu in B. circulans (WBC) to 0.912 x 102 unit/cfu in B. licheniformis(WBL) for corn starch, and 0.01 x 102 unit/cfu in both B. megaterium (SBG) and B. licheniformis (SBL) to 0.693 x 102 unit/cfu in B. subtilis (WBS) for soluble starch.

 

Key words: Activity, Bacillus, enzymatic, metabolic, starchy, substrate.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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