Abstract

The GenBank sequences of UBGAT (UDP-glucose:flavonoid 7-O-glucosyltransferase) genes and their encoding proteins from Scutellaria baicalensisand Scutellaria laeteviolacea, were predicted and analyzed by some bioinformatics methods and tools such as expasy, vector NTI, National Center for Biotechnology Information (NCBI) etc. The results obtained are as follows: both Sbubgat andSlubgat genes contained complete open reading frame (ORF); the formula of SbUBGAT and SbUBGAT were C₂₁₉₃H₃₄₆₂N₅₉₀O₆₃₈S₁₁ and C₂₂₆₃H₃₅₆₂N₅₉₆O₆₆₄S₁₃, respectively and both proteins were found in the cytoplasm, without transmembrane topological structure, and were hydrophic proteins related to intermediary metabolism and amino acid synthesis; the secondary structure of UBGATs constitute mainly α-helix and random coil and the tertiary structure were modeling. In brief, this study lay theoretical foundation for metabolic mechanism and genetic regulation researches of flavonoids biosynthesis.

Key words: Scutellaria baicalensis, Scutellaria laeteviolacea, flavonoids, bioinformatics, UDP-glucose:flavonoid 7-O-glucosyltransferase.