Comparative performance of different yeast strains in the production of bioethanol using simultaneous liquefaction saccharification and fermentation (SLSF)

Edith Lemechi Leonald-Emeka; Elizabeth Funmilayo Aransiola; Taiwo Hassan Ibrahim; Julius Olusegun Oyedele; Rose Maxwell Gidado; Andrew Chibuzor Iloh; Bamidele Ogbe Solomon

doi:10.5897/AJB2020.17089

African Journal of
Biotechnology

Abbreviation: Afr. J. Biotechnol.
Language: English
ISSN: 1684-5315
DOI: 10.5897/AJB
Start Year: 2002
Published Articles: 12486

Full Length Research Paper

Comparative performance of different yeast strains in the production of bioethanol using simultaneous liquefaction saccharification and fermentation (SLSF)

Edith Lemechi Leonald-Emeka

Edith Lemechi Leonald-Emeka
Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
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Elizabeth Funmilayo Aransiola

Elizabeth Funmilayo Aransiola
Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
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Taiwo Hassan Ibrahim

Taiwo Hassan Ibrahim
Bioresources Development Center, National Biotechnology Development Agency, Ogbomosho, Oyo State, Nigeria.
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Julius Olusegun Oyedele

Julius Olusegun Oyedele
Bioresources Development Center, National Biotechnology Development Agency, Ogbomosho, Oyo State, Nigeria.
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Rose Maxwell Gidado

Rose Maxwell Gidado
National Biotechnology Development Agency, Abuja, Nigeria.
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Andrew Chibuzor Iloh

Andrew Chibuzor Iloh
Biotechnology Advanced Research Centre, Sheda Science and Technology Complex, Abuja, Nigeria.
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Bamidele Ogbe Solomon

Bamidele Ogbe Solomon
Department of Chemical Engineering, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria.
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Article Number - FC29AF165663
Vol.19(12), pp. 858-867 , December 2020
https://doi.org/10.5897/AJB2020.17089

Received: 11 February 2020
Accepted: 20 March 2020
Published: 31 December 2020

Abstract

The performance of a hybrid of Saccharomyces cerevisiae and Saccharomyces paradoxus (Yeast B) compared with an industrial strain of S. cerevisiae (Yeast A) was studied. The two strains of yeasts: Yeast A obtained from the Bioresources Development Centre, National Biotechnology Development Agency Ogbomosho and Yeast B obtained from Scotch Whisky Research Laboratory in Edinburgh, Scotland (Strain 63M) were studied using the SLSF method for bioethanol production at various initial starch concentrations (20, 30 and 50%). The cassava starch used was extracted from Tropical Manihot esculenta (TME 419) cassava strain while the SLSF method for bioethanol production was initiated by adding granular starch hydrolysing enzyme with the yeast strains into the starch solutions at room temperature. The results obtained showed that Yeast B has higher bioethanol yield and tolerance generating 70.34, 87.34 and 120.53 ml/L compared to the Yeast A which produced 63.4, 72.73 and 112.6 ml/L at 20, 30 and 50% starch concentrations respectively. The study suggests that the hybrid strain of S. cerevisiae and S. paradoxus out performs the industrial strain and can favorably substitute or displace the industrial strains for bioethanol production.

Key words: Bioethanol, cassava, fermentation, hybrid, simultaneous liquefaction saccharification and fermentation (SLSF), yeast, Saccharomyces cerevisiae, Saccharomyces paradoxus.