African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Review

Genetic variation of twenty autosomal STR loci and evaluate the importance of these loci for forensic genetic purposes

Imad Hadi
  • Imad Hadi
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Mohammed Abdullah
  • Mohammed Abdullah
  • Department of Molecular Biology, Babylon University, Hilla City, Iraq.
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Aamera Jaber
  • Aamera Jaber
  • Institute of medico-legal in Baghdad, Ministry of Health of Iraq, Iraq.
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Cheah Yoke
  • Cheah Yoke
  • Department of Biomedical Science, University Putra Malaysia, Selangor, Malaysia.
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  •  Received: 14 June 2013
  •  Accepted: 26 February 2014
  •  Published: 12 March 2014

Abstract

The aim of this study was of twofold. One was to determine the genetic structure of Iraq population and the second objective of the study was to evaluate the importance of these loci for forensic genetic purposes. FTA® Technology (FTA™ paper DNA extraction) utilized to extract DNA. Twenty (20) STR loci and Amelogenin), including D3S1358, D13S317, Penta E, D16S539, D18S51, D2S1338, CSF1PO, Penta D, THO1, vWA, D21S11, D7S820, TPOX, D8S1179, FGA, D2S1338, D5S818, D6S1043, D12S391, D19S433 and Amelogenin amplified by using power plex21® kit. Polymerase chain reaction (PCR) products detected by genetic analyzer 3730xL then data analyzed by PowerStatsV1.2. Based on the allelic frequencies, several statistical parameters of genetic and forensic efficiency have been estimated. This includes the homozygosity and heterozygosity, effective number of alleles (n), the polymorphism information content (PIC), the power of discrimination (DP) and the power of exclusion (PE). The power of discrimination values for all tested loci was from 75 to 96%; therefore, those loci can be safely used to establish a DNA-based database for Iraq population. The high PIC values of the selected markers confirm their usefulness for genetic polymorphism studies and linkage mapping programs in human as well. The mean heterozygosity observed, is expected to have mean PIC values across the 20 loci which were 0.77, 0.81 and 0.78, respectively, indicating high gene diversity.

 

Key words:  Autosomal STR, genetic variation, Iraq, statistical parameters.

Abbreviation

Abbreviations: STRs, Short tandem repeats; SSRs, simple sequence repeats; EDTA, ethylene diamine tetraacetic acid; TE, tris-EDTA buffer; PCR, polymerase chain reaction; Ho, observed heterozygosity; He, expected heterozygosity; PD, power of discrimination; PE, probability or power of exclusion; PIC, polymorphism information content; HWE, Hardy Weinberg equilibrium; RFLP, restriction fragment length polymorphism; CDP, combined discrimination power; PI, paternity index; RMP, random match probability.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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