African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12481

Full Length Research Paper

Conditions for regeneration and transformation of Solanum tuberosum cultivars using the cotton glutathione S-transferase (Gh-5) gene

Dong Yong Shin1#, Eun Soo Seong2#, Jong Kuk Na3, Ji Hye Yoo1, Won Hee Kang4, Bimal Kumar Ghimire5, Jung Dae Lim6 and Chang Yeon Yu1*   #These authors contributed equally to this work.
1Department of Bio-resources Technology, College of Agriculture and Life Science, Kangwon National University, Chuncheon 200-701, South Korea. 2Bioherb Research Institute, Kangwon National University, Chunchon 200-701, South Korea. 3Biology Department, 208 Mueller Laboratory, Penn State University, University Park, PA 16802, USA. 4Department of Horticulture, Kangwon National University, Chunchon 200-701, South Korea. 5Department of Ethnobotany and Social Medicine Studies,Sikkim University, Gangtok- 737 102, Sikkim, India. 6Department of Herbal Medicine Resource, Kangwon National University, Samcheok 245-711, South Korea.
Email: [email protected] [email protected]

  •  Accepted: 18 August 2011
  •  Published: 31 October 2011

Abstract

Our aim was to produce multiple stress-resistant plants containing the glutathione-S-transferase (Gh-5) auxin-regulated gene, which is related to the presence of the stress resistance protein and is expressed in the transgenic plant genome. We used the Agrobacterium-mediated transformation method. In all tested cultivars (Chubaek, Jasim, Jopoong, Namjak and Sumi), there were higher regeneration frequencies from internode explants than from leaf explants. Incorporation of the GST gene into potato was confirmed by PCR analysis of genomic DNA that demonstrated the 585 bp band. Southern blot analysis confirmed the integration of the Gh-5 gene into the genomic DNA of both forms. No such bands were obtained from non-transformed potato DNA; double bands were detected in digested DNA from transformed potato plants. 

 

Key words: Glutathione-S-transferase gene (Gh-5), potato, transformation, transgenic plant.

Abbreviation

IBA, Indole-3-butyric acid; MS, Murashige & Skoog; NAA, α-naphthaleneacetic acid; GA3, gibberellic acid-3; IAA, indole acetic acid.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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