African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Isolation, partial purification and characterization of antifungal trypsin inhibitor protease from the seed of Blighia sapida K.D. Koenig (Ackee)

Nwozo S. O.*
  • Nwozo S. O.*
  • Nutritional / Industrial Biochemistry unit, Department of Biochemistry, FBMS, College of Medicine, University of Ibadan, Nigeria; SensorLab, Department of Chemistry, University of Western Cape, Bellville, Cape Town 7535, South Africa.
  • Google Scholar
Iwuoha E. I.
  • Iwuoha E. I.
  • Nutritional / Industrial Biochemistry unit, Department of Biochemistry, FBMS, College of Medicine, University of Ibadan, Nigeria; SensorLab, Department of Chemistry, University of Western Cape, Bellville, Cape Town 7535, South Africa.
  • Google Scholar
Waryo T.
  • Waryo T.
  • SensorLab, Department of Chemistry, University of Western Cape, Bellville, Cape Town 7535, South Africa.
  • Google Scholar
Kgarebe B.
  • Kgarebe B.
  • SensorLab, Department of Chemistry, University of Western Cape, Bellville, Cape Town 7535, South Africa.
  • Google Scholar


  •  Received: 25 July 2013
  •  Accepted: 08 May 2014
  •  Published: 16 July 2014

References

Bowen-Forbes CS, Minott DA (2009). Structural characterisation of hypoglcin B, a diastereomeric dipeptide from ackee fruit (Blighiasapida Koenig) by NMR experiments. MagnReson Chem. 47(11):1004-1006
Crossref
 
Bradford MM (1976). A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
Crossref
 
Cowan ST (1974). Manual for the identification of medicine - Bacteria. Cambridge University Press, London, p.8.
Pubmed
 
Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM (1989). Electrospray ionization for mass spectrometry of large biomolecules. Science. 246:64–71.
Crossref
 
Gaillard Y, Carlier J, Berscht M, Mazoyer C, Bevalot F, Guitton J, Fanton L (2011). Fatal intoxication due to ackee (Blighiasapida) in Suriname and French Guyana.GC-MS detection and quantification of hypoglycin-A.Forensic Sci Int. 206(1-3):103-107.
Crossref
 
Hassal CH, Reyle K (1954). Hypoglycin A,B: biologically active polypeptides from Blighia sapida . Nature.173 (4399): 356-357.
Crossref
 
Henzel WJ, Billeci TM, Stults JT, Wong SC, Grimley C, Watanabe C (1993). Identifying proteins from two-dimensional gels by molecular mass searching of peptide fragments in protein sequence databases.Proc. Natl. Acad. Sci. USA. 90:5011–5015.
Crossref
 
Herman EM (1994). Multiple origin of intravacuolar protein accumulation in plant cells: In K Moholtra Ed. Advances in Structural Biology. JAI press, Greenwich CT, 3:243-283.
 
Huber R, Carrell RW (1989). Implications of the three-dimensional structure of alpha I -antitrypsin for structure and function of serpins. Biochemistry. 28:8951-8966.
Crossref
 
Joskow R, Belson M, Vesper H, Backer L, Rubin C (2006). Ackee fruit poisoning: an outbreak investigation in Haiti 2000-2001 and review of the literature. ClinToxicol (Phila). 44(3):267-273.
Crossref
 
Kunitz M, McDonald MR (1946). Isolation of crystalline hexokinase and other proteins from yeast. J. Gen. Physiol. 29:143-147.
Crossref
 
Kusumoto IT, Nakabayashi T, Kida H, Miyashiro H, Hattori M,Namba T, Shimotohno K (2006). Screening of various plant extracts in ayurvedic medicine for inhibitory effect on human immunodeficiency virus type I (HIV-I) protease. Phytother Res. 9(30):180-184.
 
Lacks SA, Springhorn SS (1980). Renaturation of enzymes after polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate.J. Biol. Chem. 225:7467-7473.
 
Laemmli UK (1970).Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 227:680-685.
Crossref
 
Lam SK, Ng TB (2008). A protein with antiproliferative, antifungal and HIV-1 reverse transcriptase inhibitory activities from Caper (Capparis spinosa) seeds . Phytomedicine. 40(12):1029-1038.
 
Laskowsky M Jr., Kato I (1980). Protein inhibitors of proteinases.Annu. Rev. Biochem. 49:593–626.
Crossref
 
Lawrence PK, Koundal KR (2002). Plant protease inhibitors in control of phytophagous insects.Electron J. Biotechn. 5:93-109.
Crossref
 
Liener IE (1996). Effects of processing on antinutritional factors in legumes: the soybean case. Arch. Latinoam. Nutr. 44(4):48S–54S.
Pubmed
 
Macedo MLR, Mello GC, Freire MGM, Novello JC, Marangoni S, Matos DGG (2002). Effect of trypsin inhibitor from Dimorphan dramollis seeds on the development of Callosobruchus maculatus. Plant Pysiol. Biochem. 40:891-898.
 
Macedo ML, De Sa CM, Freire MG, Parra JRP (2004). A Kunitz-type inhibitor of Cleopteran proteases, isolated from Adenanthera pavonina L seeds and its effect on Callosobruchus masculatus. J. Agric Food Chem. 52(9):2533-2540.
Crossref
 
Macedo MLR, Xavier-Filho J (1992). Purification and characterization of trypsin inhibitors from seeds of Clitoria teratea. J. Sci. Food Agric. 58:55-58.
Crossref
 
Moya J (2001). Ackee (Blighia sapida) poisoning in the Northern Province, Haiti 2001. Epidermiol Bull. 22(2):8-9.
Pubmed
 
Murdock LL, Brookhart G, Dunn PE, Foard DE, Kelley S (1987). Cysteine digestive proteinases in Coleoptera. Comparative Biochemistry and Physiology-B, 87:783-787.
Crossref
 
Natalini B, Capodiferro V, De Luca C, Espinal R (2000). Isolation of pure (2S, '1S, 2'S)-2-(2'-carboxycyclopropyl) glycine from Blighia sapida (Ackee). J. Chromatogr. A. 873(2):283-286.
Crossref
 
O'Farrell PH (1975). High resolution two-dimensional electrophoresis of proteins.J. Biol. Chem. 250:4007–4021.
Pubmed
 
Oladiji AT, Shoremekun KL, Yakubu MT (2009). Physicochemical properties of the oil from the fruit of Blighia sapida and toxicological evaluation of the oil-based diet in wistar rats. J. Med. Food 12(5):1127-1135.
Crossref
 
Pfleinderer G, Krauss A (1965). Spectrophotometric assay for the detection of protease activity. Biochemische Zeitschrift 85:342-344.
 
Reeves DS, Phillips I, William JD (1978). Laboratory methods in antimicrobial chemotherapy. Longman Group Ltd., Edinburgh p.20.
 
Richardson M (1991). Seed storage proteins: the enzyme inhibitors. In Methods in Plant Biochemistry, Amino Acids, proteins and Nucleic Acids; Rogers, J.L. Ed.; Academic Press: New York 5:259 – 305.
 
Schumacher GFB, Schill WB (1972). Radial diffusion in gel for micro determination of enzymes: II. Plasminogen activator, elastase, and nonspecific proteases. Anal. Biochem. 48:9–26.
Crossref
 
Sharma S, Yacavone MM, Cao X, Samuda PM, Cade J, Cruickshank K (2009). Nutritional composition of commonly consumed composite dishes for Afro-Caribbean (mainly Jamaicans) in the United Kingdom. Int. J. Food Sci. Nutr. 1:1-11.
 
Shih CY, Khan AA, Jia Si, Wu J, Shih DS (2001). Purification, characterization and molecular cloning of chitinase from the seeds of Beninca sahispida. Biosci. Biotechnol. Biochem. 65(3):501-509.
Crossref