African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

A method of rapid in vitro proliferation and morphological characterization of the medicinal plant Aloe vera L.

Chandra Sekhar Singh B.
  • Chandra Sekhar Singh B.
  • Department of Plant Science, College of Agriculture and Ambo University, Ambo, Post Box : 19, Ethiopia.
  • Google Scholar
Diriba Adugna A.
  • Diriba Adugna A.
  • Department of Plant Science, College of Agriculture and Ambo University, Ambo, Post Box : 19, Ethiopia.
  • Google Scholar
Roja Rani A.
  • Roja Rani A.
  • Department of Genetics and Biotechnology, Osmania University, Hyderabad-500007, India.
  • Google Scholar


  •  Received: 12 September 2017
  •  Accepted: 30 October 2017
  •  Published: 22 November 2017

References

Abrie AL, Staden JV (2001). Micro propagation of endangered Aloe polyphylla. J. Plant Growth Regulation. 33:19-23.
Crossref

 

Aggarwal D, Barna KS (2004). Tissue culture propagation of elite plant of Aloe vera (L.) J. Plant Biochem. Biotechnol. 13:77-79.
Crossref

 
 

Ahmed S, Kabir AH (2007). Development of Rapid Micro propagation Method of Aloe vera L. Sjemenarstvo. 24:121-128.

 
 

Albanyl NJ, Vilche S, Lion MM, Chacin P (2006). A methodology for the propagation in edge Aloe vera. Rev. Fac. Agron. 23:213-222.

 
 

Baksh R, Jahan MA, Khatun R, Munshi JL (2005). Micropropagation of Aloe barbadensis Mill through in vitro culture of shoot tip explants. Plant Tissue Cult. Biotech. pp. 121-126.

 
 

Balraj Singh, Neelu Sood (2009). Significance of explants preparation and sizing in Aloe vera (L.). A highly efficient method for in vitro multiple shoots induction. Scientia Horticulturae. 122:146-151.
Crossref

 
 

Bhandari, Aravind Kumar, Negi, JS, Bisht VK, Bharti MK (2010). In vitro propagation of Aloe vera - A plant with Medicinal properties. Nat. Sci. 8:8

 
 

Bhojwani SS (1980). In vitro propagation of garlic by shoot proliferation. Sci. Hortic. 13:47-52.
Crossref

 
 

Bhojwani SS, Razdan MK (1992). Plant Tissue Culture Theory and Practice. Elsevier, Amsterdam, London, New York, Tokyo Biol. Sci. 39: 1, 1- 9

 
 

Budhiani E (2001). Micropropagation of Aloe vera through shoot multiplication. Ph.D. Thesis. Indonesia DLN Institute. Indonesia.

 
 

Campestrini LH, Kuhnen S, Lemos PMM, Bach DB, Dia PF, Maraschin M (2006). Cloning protocol of Aloe vera as a study-case for "Tailor-Made" Biotechnology to small farmers. J. Technol Manag Innov. 1(5):76-79.

 
 

Chandra Sekhar SB, Rama Rao B, Adhrvana Chari M, Chakrapani P, Latha T, Venkatesh K, Arun Jyothi B, Amareswari P, Roja Rani A (2013). Cultivation, Phytochemical studies, Biological activities and Medicinal uses of Aloe ferox grand father of aloes an important medicinal plant. Int. J. Pharmacol. 9(7):405-415.
Crossref

 
 

Chaudhuri S, Mukundan U (2001). Aloe vera (L.) micropropagation and characterization of its gel. Phytomorphol. 51(2):155-157.

 
 

Debiasi C, Silva C, Pescador R (2007). Micropropagation of Aloe vera L. Revista Brasileira De Plantas Medicinais. 9:36-43.

 
 

Dwivedi NK, Indiradevi A, Asha KI, Asokan Nair R, Suma A (2014). A protocol for micro propagation of Aloe vera L. (Indian Aloe) – a miracle plant. Res. Biotechnol. 5(1):1-5.

 
 

Gantait S, Mandal N, Bhattacharya S, Das PK (2010). A Novel Strategy for in vitro conservation of Aloe vera L. through long term shoot culture. Biotechnology. 9(3):326-331.
Crossref

 
 

Garland P, Stoltz LP (1981). Micropropagation of Pissrdi plum. Ann Bot. 48:387-389.
Crossref

 
 

Gui YL, Xu TY, Gu SR, Liu SQ, Zhang Z, Sun GD, Zhang Q (1990). Studies on stem tissue culture and organogenesis of Aloe vera. Acta Bot Sini. 32(8), 606-610.

 
 

Groenewald EG, Koeleman A, Wessels DCJ (1975). Callus formation and plant regeneration from seed tissue of Aloe pretoriensis. Zeitschrift für Pflanzenphysiologie, 75(3), 270-272.
Crossref

 
 

Hashemabadi D, Kaviani B (2008). Rapid micro-propragation of Aloe vera L. via shoot multiplication. Afr. J. Biotechnol. 7(12):1899-1902.
Crossref

 
 

Hirimburegama K, Gamage N (1995). In vitro multiplication of Aloe vera meristem tips for mass propagation. Hort. Sci. 27: 15-18.

 
 

Hosseini R, Parsa M (2007). Micropropagation of Aloe vera L. grown in South Iran. Pakis J. Biol. Sci. 10 (7):1134-1137.
Crossref

 
 

Liao Z, Chen M, Tan F, Sun X, Tang K (2004). Micro propagation of endangered Chinese Aloe. Plant Cell Tiss. Org. Culture. 76(1):83-86.
Crossref

 
 

Mangal Singh Rathore MS, Panvar D, Rathore JSH, Dagla R, Shekhawat NS (2009). Micropropagation of selected genotypes of Aloe vera (L) An ancient plant for modern Industry. J. Sustain. For. 28:935-950.
Crossref

 
 

Meyer HT, Staden JV (1991). Rapid in vitro propagation of Aloe barbadensis Mill. Plant Cell Tissue Organ Cult. 26:171-176.
Crossref

 
 

Mukesh Kumar, Sumer Singh, Satyapal Singh (2011). In vitro Morphogenesis of a medicinal plant - Aloe vera L. Asian J. Plant Sci. Res, 1(1), 31-40.

 
 

Mukherjee A, Roy Chowdhury B (2008). The in vitro propagation of Aloe vera sp. TIG Res. J. 1(2):116-119.

 
 

Murashige T, Skoog F (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant. 15: 473-479.
Crossref

 
 

Natali L, Sanchez IC, Cavallini A (1990). In vitro culture of Aloe barbadensis Mill: micropropagation from vegetative meristems. Plant Cell Tiss. Org. Cult. 20: 71-74.
Crossref

 
 

Racchi ML (1987). Plant regeneration from callus cultures of Aloe ferox. Proc. Int. Congr. Plant tissue culture, Bogota.

 
 

Radha Mahavi YR, Swapna RS, Pradeepkiran JA, Ismail SM, Madhuri E, Bhaskar M (2012). Protective role of ethanolic extract of Aloe vera anti-oxidant properties on liver and kidney of streptozotocin- induced diabetic rats. Digest J. Nano Materials Bio-structures. 7:175 -184.

 
 

Rajasekaran S, Sivagnanam K, Ravi K, Subramanian S (2005). Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacol Rep. 57:90-96.

 
 

Reynolds T (2004). Aloes -The genus Aloe. Medicinal and Aromatic plants Industrial profiles USA 38.

 
 

Richwine AM, Tipton JL, Thompson GA (1995). Establishment of Aloe Gasteria and Hawothia shoot cultures from inflorescence explants. Hort Science. 30(7):1443-1444.

 
 

Rines HW, Mc Coy TJ (1981). Tissue culture initiation and plant regeneration in hexapody species of oats. Crop Sci. 21:837-842.
Crossref

 
 

Roy SC, Sarkar A (1991) In vitro regeneration and micropropagation of Aloe vera L. Sci Hort 47: 107-113.
Crossref

 
 

Rout GR, Reddy GM, Das P (2001). Study on in vitro clonal propagation of Paulownia tomentosa Steud evaluation of genetic fidelity through RAPD Marker. Silvae Genet. 50:208-212.

 
 

Sanchez IC, Natali L, Cavallini A (1988). In vitro culture of Aloe barbadensis Mill. Morphogenetic ability and nuclear DNA content. Plant Sci 55: 53-59.
Crossref

 
 

Sibi M (1982). Heritable epigenetic variation from in vitro tissue culture of Lycopersian esculentum (Var. Monalbo) In: E.D. Earle and Y. Demarly (Editors). Variability in plants regenerated from tissue culture. Proc NSFCNRS Congr.Osray.Prager, New York. 228-244.

 
 

Silva-Pereira LC, Cardoso PC, Leite DS, Bahia MO, Bastos WR, Smith MA (2005). Cytotoxicity and Genotoxicity of low doses of mercury chloride and methyl mercury chloride on human lymphocytes in vitro. Brazilian J. Med. Biol. Res. 901-907.
Crossref

 
 

ShekhRahman, Princeton Carter, Narayan Bhattarai (2017). Aloe vera for Tissue Engineering Applications. J. Funct Biomater. 8:6.
Crossref

 
 

Tripathi BK, Bitallion C (1995) In vitro regeneration of Hedychium roxburgii blume through rhizome meristem culture. Hort Sci. 4:11-17.

 
 

Velcheva M, Faltin Z, Vandi A, Eshdat Y, Perl A (2005).Regeneration of Aloe arborescens via organogenesis from young inflorescences. Plant Cell Tiss Org Cult. 83:293- 301.
Crossref

 
 

Walkey DGA, Nelly HA, Crisp P (1980). Rapid propagation of white cabbage by tissue culture. Sci Hort. 12: 2:99-107.
Crossref

 
 

Werker E, Leshem B (1987). Structural changes during vitrification of carnation plantlets. Ann Bot. 59:377-385.
Crossref

 
 

World Health Organization (WHO) (2005). Mercury in drinking water. 

View

 
 

Williams LD, Burdock GA, Shin E, Kim S Jo TH, Jones KN, Matulka RA (2010). Safety studies conducted on a proprietary high-purity Aloe vera inner leaf fillet preparation, Qmatrix. Regul Toxicol Pharmaco. 57:90-98.
Crossref

 
 

Yagi A, Shoyama Y, Nishioka I (1983). Formation of tetra hydro anthracene glucosides by callus tissue of Aloe saponaria. Phytochem. 22:1483-1484.
Crossref

 
 

Yagi A, Sato Y, Shimomura K, Akasaki K, Tsuji H (2000). Distribution of verectin in Aloe vera leaves and verectin contents in clonally regenerated plants and the commercial gel powders by immunochemical screening. Planta Med. 66:180-182.
Crossref

 
 

Yagi A, Sato Y, Miwa Y, Kabbash A, Moustafa S, Shimomura K and El-Bassuony A. (2006). Ribosomal DNA sequence analysis of different geographically distributed Aloe vera plants: comparison with clonally regenerated plants. Saudi Pharm. J. 14(3-4):208-211.

 
 

Zhou G, Ding H, Shi W, Cheng L (1999). Fast asexual propagation of Aloe vera. Horticul. 26 (6):410-411.