African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Review

Reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) and its usefulness in soil microbial ecological studies - A review

Stella Asuming-Brempong
  • Stella Asuming-Brempong
  • Department of Soil Science, College of Agriculture and Consumer Sciences, University of Ghana, Legon, Ghana.
  • Google Scholar


  •  Received: 13 December 2013
  •  Accepted: 10 January 2014
  •  Published: 05 February 2014

References

Arriano CM (1993). Post-transcriptional control of gene expression: bacterial mRNA degradation. World J. Microbiol. Biotechol. 9(4):421-432.
Crossref
 
Baelum J, Henriksen T, Hansen HCB, Jacobsen CS (2006). Dedgra-dation of 4-chloro-2-methylphenoxyaqcetatic acid in top and subsoil is quantitatively linked to the class III tfdA gene. Appl. Environ. Microbiol. 72(2):1476-1486.
Crossref
 
Baelum J, Nicolaisen MH, Holben WE, Strobel BW, Sorensen J, Jacobsen CS (2008). Direct analysis of tfdA gene expression by indigenous bacteria in phenoxy acid amended agricultural soil. Int. Soc. Microb. Ecol. 2(45):667-687.
 
Bornemann J, Triplett EW (1997). Rapid and direct method for extraction of RNA from soil. Soil Biol. Biochem. 29(11-12):1621-1624.
Crossref
 
Botero LM, D'Imperio S, Burr M, McDermott TR, Young M, Hassett DJ (2005). Poly (A) polymerase modification and reverse transcriptase PCR amplification of environmental RNA. Appl. Environ. Microbiol. 71(3):1267-75.
Crossref
 
Burgmann H, Pesaro M, Widner F, Zeyer J (2001). A strategy for optimizing quality and quantity of DNA extracted from soil. J. Microbiol. Methods 45(1):7-20.
Crossref
 
Burgmann H, Widmer F, Sigler WV, Zeyer J (2003). mRNA extraction and reverse transcription-PCR protocol for detection of nifH Gene expression by Azotobacter vinelandii in soil. Appl. Environ. Microbiol. 69(4):1928-1935.
Crossref
 
Bustin SA (2000). Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J. Mol. Endocrinol. 25 (2):169-193.
Crossref
 
Bustin SA, Benes V, Nolan T, Pfaffl MV (2005). Quantitative real-time RT-PCR perspective. J. Mol. Endocrinol. 34(3):597-601.
Crossref
 
Cheung AL, Eberhardt KJ, Fischetti VA (1994). A method to isolate RNA from gram positive bacteria and mycobacteria. Anal. Biochem. 222(2):511-514.
Crossref
 
DeCoste NJ, Gadkar VJ, Filion M (2011). Relative and Absolute Quantitative Real-Time PCR-Based Quantifications of hcnC and phlD gene transcripts in natural soil spiked with Pseudomonas sp. strain LBUM300. Appl. Environ. Microbiol. 77(1):41- 47.
Crossref
 
Devers M, Soulas G, Martin-Laurent F (2004). Real-time reverse transcription PCR analysis of Expression of atrazine Catabolism Genes in two bacterial strains isolated from Soil. J. Microbiol. Methods 56(1):3-15.
Crossref
 
Eleaume H, Jabbori S (2004). Comparison of two standardization methods in real-time quantitative RT-PCR to follow Staphylococcs aureus genes expression during in vitro growth. J. Microbiol. Methods 59(3):363-370.
Crossref
 
England LS, Trevors JT (2003). The microbial DNA cycle in soil. Biol. Forum 96(2):249-258.
 
England LS, Holmes SB, Trevors JT (2001). extraction and detection of baculoviral DNA from lake water detritus and forest litter. J. Appl. Microbiol. 90(4):194-205.
Crossref
 
Farrel RE (1998). RNA Methodologies. 2nd ed. Academic Press, San Diego, Calif.
 
Fleming T, Yao WH, Sayler GS (1998). Optimization of differential display of prokaryotic mRNA: Application to pure culture and soil microcosms. Appl. Environ. Microbiol. 69(10):3698-3706.
 
Freitag TE, Prosser JI (2009). Correlation of Methane Production and Functional gene Transcriptional Activity in a Peat Soil. Appl. Environ. Microbiol. 75 (21)P:6679-6687.
Crossref
 
Gruntzig V, Nold SC, Zhou J, Tiedje JM (2001). Pseudomonas stutzeri nitrite reductase gene abundance in environmental samples measured by real-time PCR. Appl. Environ. Microbiol. 67(2):760-768.
Crossref
 
Gottschal JC, Meijer WG, Oda Y (1997). Use of molecular probing to assess microbial activities in natural ecosystems. Microbial Communities, (ed. Insam H, Ranger A), Springer, Heidelberg. pp. 10-18.
Crossref
 
Hurt RA, Qui X, Wu L, Roh Y, Palumbo AV, Tiedje JM, Zhou J (2001). Simultaneous recovery of RNA and dna from soils and sediments. Appl. Environ. Microbiol. 67:4495-4503.
Crossref
 
Heid CA, Stevens J, Livak KJ, Williams PM (1996). Real-time quant-itative PCR. Genome Res. 6:986-984.
Crossref
 
Johnson DR, Lee PKH, Holmes VF, Alvares-Cohen L (2005). An internal reference technique for accurately quantifying specific mRNAs by real time PCR with application to the tceA reductive dehalogenase gene. Appl. Environ. Microbiol. 71(7):3866-3871.
Crossref
 
Kreader C (1996). Relief of Amplification Inhibition in PCR with Bovine Serum Albumin or T4 gene 32 Protein. Appl. Environ. Microbiol. 62 (3):1102-1106.
Pubmed
 
Lindahl T (1993). Instability and Decay of the Primay Structure of DNA", Nature 362(6422):709-715.
Crossref
 
Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta C(T)) method. Methods 25(4):402-408.
Crossref
 
Mendum TA, Sockett RE, Hirsch PR (1998). The detection of Gram-negative bacteria mRNA from soil by RT-PCR. FEMS Microbiol. Lett. 164 (2):369-373.
Crossref
 
Miskin I, Rhodes G, Lawlor K, Saunders JR Pickup RW (1998). Bacteria in post-glacial freshwater sediments. Microbiology 144(9):2427-2439.
Crossref
 
Novinscak A, Filion M (2011). Effect of soil clay content on RNA isolation and on detection and quantification of bacterial gene transcripts in soil by quantitative reverse transcription-PCR. Appl. Environ. Microbiol. 77(17):6249-6252.
Crossref
 
Ogram A, Sun W, Brockman FJ, Fredrickson JK (1995). Isolation and characterization of RNA from low-biomass deep-surface sediments. Appl. Environ. Microbiol. 61(2):763-768.
Pubmed
 
Sayler GS, Flemming JT, Nivens DE (2001). Gene expression monitoring in soils by mRNA analysis and gene lux fusions. Curr. Opin. Biotechnol. 12(5):455-460.
Crossref
 
Schmittegen TD, Zakrajsek BA (2000). Effect of experimental treat-ment on Housekeeping Gene Expression: Validation by real-time, quantitative RT-PCR. J. Biochem. Biophys. Methods 46(1-2):69-81.
Crossref
 
Sessitsch A, Gyamfi S, Stralis -Paverse N, Weilharter A, Pfeifer U (2002). RNA isolation from soil for bacteria community and functional analysis: Evaluation of different extraction and soil conservation protocol. J. Microbiol. Methods 51:171-179.
Crossref
 
Tao H, Bausch C, Richmond C, Blattner FR, Conway T (1999). Functional genomics: Expression analysis of Escherichia coli growing on minimal and rich media. J. Bacteriol. 181(20):6425-6440.
Pubmed
 
Thellin O, Zorzi W, Lakaye B, De Bornman B, Coumans B, Hennen G, Grisar T, Igou TA, Heinen E (1999). Housekeeping genes as internal standards: Use and limits. J. Biotechnol. 75:291-295.
Crossref
 
Torsvik VL, Goksoyr J, Daae FL (1990). High Diversity in DNA of soil bacteria. Appl. Environ. Microbiol. 56(3):782-787.
Pubmed
 
Treusch AH, Leininger S, Kletzin A, Schuster SC, Klenk HP, Schleper C (2005). Novel genes for nitrite reductase and amo-related proteins indicate role of uncultivated mesophilic crenarchoeta in nitrogen cycling. Environ. Microbiol. 7(12):1985-1995.
Crossref
 
Trevors JT (1996). Nucleic acids in the environment. Curr. Opin. Biotechnol. 7(3):331-336.
Crossref
 
Tsai YL, Pasrk MJ, Olson BH (1991). Rapid method for direct extraction of mRNA from seeded soil. Appl. Environ. Microbiol. 57(3)P:765-768.
Pubmed
 
Wang T, Brown MJ (1999). mRNA Quantification by real time TaqMan polymerase chain reaction: Validation and comparison with RNase protection. Anal. Biochem. 269(1):198-201.
Crossref