June 2014
Micrografting for fruit crop improvement
Micrografting is an in vitro grafting technique which involves the placement of a meristem or shoot tip explant onto a decapitated rootstock that has been grown aseptically from seed or micropropagated cultures. Following early experiments of micrografting in ivy and chrysanthemum, the technique has been used in woody species, especially fruit trees. Major work was carried out in different Citrus species for the...
June 2014
Identification and cloning of two insecticidal protein genes from Bacillus thuringiensis strain S185
Bacillus thuringiensis (Bt) is the most widely applied type of microbial pesticide due to its high specificity and environmental safety. The activity of Bt is largely attributed to the insecticidal crystal protein encoded by the cry genes. Different insecticidal crystal proteins of Bt have different bioactivity against distinct agricultural insect pests, and combination of these proteins not only increases insecticidal...
June 2014
Molecular characterization of African swine fever virus in apparently healthy domestic pigs in Uganda
African swine fever (ASF) is a highly lethal and economically significant disease of domestic pigs in Uganda where outbreaks regularly occur. There is neither a vaccine nor treatment available for ASF control. Twenty two African swine fever virus (ASFV) genotypes (I - XXII) have been identified based on partial sequencing of the C-terminus of the major capsid protein p72 encoded by the B646L gene. The majority of...
June 2014
In vitro propagation of the elite species plant Pluchea lanceolata: Assessment of genetic stability by random amplified polymorphic DNA (RAPD) analysis
An effective in vitro regeneration protocol was developed from nodal segment of Pluchea lanceolata (DC.) Oliver. & Hiern, a medicinally important plant used in ayurvedic system of medicine for curing diseases similar to rheumatoid arthritis. Nodal segments were cultured in MS medium supplemented with auxin and cytokinin and their combinations. The objective was to produce genetically identical plants, via multiple...
June 2014
Relative expression of genes related with cold tolerance in temperate rice at the seedling stage
Low temperature is one of the main abiotic stresses affecting rice yield in Chile. Alterations in phenology and physiology of the crop are observed after a cold event. The objective of this work was to study the relative expression of genes related with cold stress in Chilean cultivars of rice. For this, we analyzed the expression of candidate genes using real-time polymerase chain reaction (rtPCR), after exposure to...
June 2014
Effect of plant growth regulators on regeneration of the endangered medicinal plant Calligonum comosum L. Henry in the Kingdom of Bahrain
The use of in vitro techniques for conserving plant biodiversity and protecting rare and endangered multipurpose plant species is considered as one of the most important ex-situ conservation policies. Development of an efficient in vitro regeneration protocol of Calligonum comosum is important and that has achieved to protect the endangered multipurpose medicinally important desert plant in the Kingdom of Bahrain. Nodal...
June 2014
Rock phosphate solubilization by the ectomycorrhizal fungus Laccaria fraterna and its associated mycorrhizal helper bacterial strains
Phosphorus (P) is mostly present in the form of insoluble phosphates in soil and so plants cannot utilize it. Plant roots are often associated with mycorrhizal fungi and other microorganisms that effectively can absorb P and other elements from the soil. In the present study, few mycorrhizal helper bacterial (MHB) strains such as Pseudomonas putida, Erwinia herbicola and Bacillus subtilis were isolated and identified...
June 2014
Optimization of mutanase production by Trichoderma harzianum
The present paper describes optimization of fermentation conditions in shaken flasks and scale-up of fermentor production up to 115 L. The response surface methodology (RSM) has been successfully applied in standardization of mutanase production by Trichoderma harzianum CCM F-340. The model was very well fitted to the experimental data and explained more than 96% of the whole variation of the response (adjusted R2...
June 2014
Characterization of effective bio-control agent Bacillus sp. SRB 27 with high salt tolerance and thermostability isolated from forest soil sample
A potential antagonist Bacillus sp. SRB 27, against Fusarium oxysporum, Rhizoctonia solani and Alternaria solani was obtained from forest soil sample by carrying out in vitro and in vivo screening techniques. This study reports the identification and characterization of a Bacillus sp. SRB 27 that may be used as a bio-control agent against the plant diseases in crop plants. It was identified as Bacillus sp. SRB 27 based...
June 2014
Isolation and identification of antimicrobial-producing lactic acid bacteria from fermented cucumber
Lactic acid bacteria (LAB) responsible for spontaneous fermentation of cucumber were isolated and their antimicrobial producing potentials were screened against 10 indicator strains. 65% of the isolated LAB produced antimicrobial activities against at least two indicator strains. The indicator strains used were: Escherichia coli, Bacillus licheniformis, B. cereus, Proteus species, Staphylococcus aureus, Salmonella...
June 2014
Acceptability of prebiotic fiber-treated whey drink fermented with Lactobacillus acidophilus
This work aimed to develop a fermented drink by diversifying the quantities of Lactobacillus acidophilus inoculum and prebiotic fiber in the form of inulin and using the total dry extract of whey and sucrose. After fermentation, the following measurements were made after 0, 7, 14, 21 and 28 days of storage at 6°C: titratable acidity (according to the Dornic method), pH, moisture content, fat content, ash content,...
June 2014
Impact of pineapple waste silage on intake, digestibility and fermentation patterns of West African dwarf sheep
Pineapple waste (PW) is a by-product from pineapple processing, mostly dumped to pollute the environment. This study was conducted to recycle PW by ensiling with maize cobs and brewer dried grains at different levels. Twelve (12) West African dwarf rams weighing between 15.00 to16.10 kg were randomly distributed into four diets. Diets included: 0, 20, 40 and 60% PW as diets 1, 2, 3 and 4, respectively. Parameters...
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