June 2024
Modifications of growth, mineral uptake, chlorophyll content, osmolyte contents, antioxidant compounds and yield of three varieties of yam (Dioscorea rotundata L.) in saline conditions
A vast area of the world is negatively impacted by salinity which causes loss of crop yields. Modifications in the growth, mineral uptake, chlorophyll content, osmolyte contents, antioxidant compounds and yield of three varieties of yam (Dioscorea rotundata L.) were evaluated in saline conditions, in a greenhouse (0, 50, 100 and 200 mM) and farm (0 and 50 mM). The leaf area, stem height, noose diameter, chlorophyll...
June 2024
Assessment and validation of reference genes for qRT-PCR normalization in local cowpea (Vigna unguiculata L.) varieties in response to sterilized and unsterilized soil conditions
Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is widely employed as a mandatory analysis in biological work to analyze and validate gene expression; however, its precision is greatly hampered by the robustness of the internal control genes employed for normalization. In this study, we selected suitable housekeeping genes in leaves of cowpea for normalization of expression analysis when...
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