African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: 20 December 2012; 11(102)

December 2012

Isolation and molecular identification of β-carotene producing strains of Dunaliella salina and Dunaliella bardawil from salt soil samples by using species-specific primers and internal transcribed spacer (ITS) primers

  Dunaliella salina and Dunaliella bardawil are unique species of the genus Dunaliellathat produce large amounts of β-carotene when cultivated under appropriate conditions. These include high light intensity, high sodium chloride concentration, nitrate deficiency and extreme temperatures. Under these conditions, only D. salinaand D. bardawil can accumulate β-carotene...

Author(s): Ramaraj Sathasivam, , Prasart Kermanee, Sittiruk Roytrakul and Niran Juntawong, ,

December 2012

The effect of plant density with different row spacing on quality of the fatty acid composition and grain yield of sunflower

  This research was aimed to assess the influence of density with different row spacingon sunflower crop in two different locations in southern Italy. The experiment was laid out in a randomized block design with four replicates. It involved the comparison of sunflower grown in the field on 25 m2 (5 x 5 m) plots at three plant densities (7.5, 5.0...

Author(s): Cucci, G., Rotunno, T., Lacolla, G. and Di Caterina, R.

December 2012

Comparison between random amplified polymorphic DNA (RAPD) and simple sequence repeat (SSR) markers with high resolution melt analyses in genetic variation analysis among selected sorghum genotypes

  Understanding the genetic diversity of germplasm is essential in plant breeding programmes and germplasm management. Molecular markers are efficient and effective tools widely used for assessing genetic diversity among crop genotypes. Recently, high resolution melt analysis (HRM) has been reported for detecting genetic variability. However, there is limited information on the use of HRM in conjunction with...

Author(s): M. A.  Mofokeng, G. Watson, H. Shimelis and P. Tongoona

December 2012

Making high speed diesel and motor gasoline more environmental friendly using Dalbergia sissoo wood powder

  The present study aimed at removing chromium (Cr), nickel (Ni) and zinc (Zn) from high speed diesel and motor gasoline samples using wood powder of Dalbergia sissoo. Physical parameters of the samples, such as colour, density, specific gravity viscosity, refractive index, specific refractive index, electronic polarizibility, dipole moment and volatile contents were measured using standard methods before...

Author(s): Khizar Ahmad, Ghulam Yasin, Farzana Mahmood and Muhammad Ashraf

December 2012

Effect of the presence of surfactant and ionic liquids on the esterification of oleic acid catalyzed by immobilized lipase

  Ionic liquids (ILs) and surfactants are widely used in order to increase the catalytic efficiency of lipases. In this sense, the present study aimed to evaluate the potential of different ionic liquids (C4MIM.Ac, C4MIM.HSO4, C4MIM.TF2N, C4MIM.BF4, C12MIM.I, and C12MIM.Cl), Aliquat 336 and di-2-ethylhexyl sodium sulfosuccinate (AOT) as adjuvants in the esterification of oleic acid with different alcohols...

Author(s):   M. L. B. Queiroz, H. M. Alvarez, R. F. Boaventura, N. B. Carvalho, C. M. F. Soares, A. S. Lima, M. F. Heredia, C. Dariva, and A. T. Fricks,  

December 2012

Free radical scavenging and cytotoxic activity of five commercial standardized extracts (red wine, green tea, pine bark, polygonum and pomegranate)

  The objective of extract standardization is to obtain optimum and consistent quality of herbal preparations with defined components. This paper presents the radical scavenging effect of extracts against DPPH free radical (2,2-diphenyl-1-picrylhydrazyl) and cytotoxic activity in vitro on the cervical cancer cell line HeLa of five commercial standardized extracts rich in phenolic compounds: red wine...

Author(s): García-Becerra Ledy, Leos-Rivas Catalina, Oranday-Cárdenas Azucena and Rivas-Morales Catalina

December 2012

Development and characterization of nine polymorphic microsatellite markers in the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae)

  In this study, nine microsatellite loci were isolated and characterized from the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae). The loci were validated and characterized using 20 samples collected from five Korean localities. These results indicate that some loci were highly variable in terms of number of alleles (2 to 13), heterozygosity (0.10 to 0.40), and...

Author(s):   Min Jee Kim, Ki-Hwan Kim, Kee Young Kim, Youngho Cho and Iksoo Kim