African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Full Length Research Paper

Development and characterization of nine polymorphic microsatellite markers in the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae)

  Min Jee Kim1, Ki-Hwan Kim2, Kee Young Kim3, Youngho Cho4 and Iksoo Kim1*  
  1College of Agriculture and Life Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea. 2Bionics, 707 Hansin IT-TOWER, 235, Guro3-dong, Guro-gu, 152-768, Seoul, Republic of Korea. 3Department of Agricultural Biology, National Academy of Agricultural Science, Suwon 441-100, Republic of Korea. 4Department of Biology, Daejeon University, Daejeon 300-716, Republic of Korea.  
Email: [email protected]

  •  Accepted: 26 November 2012
  •  Published: 20 December 2012

Abstract

 

In this study, nine microsatellite loci were isolated and characterized from the seven-spotted lady beetle, Coccinella septempunctata (Coleoptera: Coccinellidae). The loci were validated and characterized using 20 samples collected from five Korean localities. These results indicate that some loci were highly variable in terms of number of alleles (2 to 13), heterozygosity (0.10 to 0.40), and polymorphic information content (0.31 to 0.85). These microsatellite markers will be very valuable for population genetic studies of C. septempunctata.

 

Key words: Seven-spotted lady beetle, Coccinella septempunctata, microsatelliteDeoxyribonucleic acid (DNA).

Copyright © 2024 Author(s) retain the copyright of this article.

This article is published under the terms of the Creative Commons Attribution License 4.0

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