October 2015

Comparison of anther and microspore culture in androgenic embryogenesis and regeneration of broccoli (Brassica oleracea L. var. italica P.)

The aim of this study was to compare the efficiency of broccoli anther and microspore culture methods for doubled haploid (DH) lines production. We evaluated the main influencing factors and optimized the culture methods to improve embryo induction and plant regeneration for efficient doubled haploid production in broccoli breeding. Six broccoli hybrids were used in this study. Our results show that generally, the...

Author(s): Ying Qin, Yunyu Huang, Seppo Pulli and Yang-Dong Guo

October 2015

Suppressive effects of a polymer sodium silicate solution on powdery mildew and root rot diseases of miniature rose

Sodium silicate was dissolved in water in either a monomer form or polymer form; the effects of both forms of sodium silicate aqueous solution on rose powdery mildew and root rot diseases of miniature rose were examined. Both forms of sodium silicate aqueous solution were applied to the roots of the miniature rose. Potassium silicate aqueous solution was used as a control and was compared to the effect of sodium...

Author(s): Mohsen Mohamed Elsharkawy, Tatsuya Hase, Masafumi Shimizu and Mitsuro Hyakumachi

October 2015

Toxic effects of Ricinus communis non-protein trypsin inhibitor on Spodoptera frugiperda (J. E. Smith) (Lepidoptera:Noctuidae)

In the study reported herein, we aimed to isolate a trypsin inhibitor from Ricinus communis leaves through chromatographic and spectrometric techniques and evaluate its toxic effects on the development of Spodoptera frugiperda larvae. Plant extracts were submitted to fractionation in adsorption column. The fraction 10, which showed the highest inhibitory activity, were incorporated into an artificial diet at the...

Author(s): Gislaine A. Carvalho, Custódio D. dos Santos, Dejane S. Alves, Geraldo A. Carvalho, Maria das G. Cardoso and Marcelo M. de Haro

October 2015

The efficacy of palm oil sludge in reducing ruminal methanogenesis using rumen simulation technique

The effect of including palm oil sludge in cattle diet (silage) was conducted in vitro in order to ascertain the suppressing effect on ruminal methanogenesis. The silage used for the study was Panicum maximum, Centrocema pubscens, Bracharia decumbens and Andropogon gayanus. The silage were cut into an average size of 3 to 5 cm and ensiled for 21 days. Four treatment diets were formulated with the silage by including...

Author(s): L. C. Ugwuowo, A. G. Ezekwe, A. O. Ani, S. I. Eze, C. N. Anyanwu and A. Ofomatah