April 2015
Genetic diversity analysis of mustard (Brassica spp.) germplasm using molecular marker for selection of short duration genotypes
Molecular characterization of 16 mustard (Brassica spp.) genotypes by using 12 RAPD markers revealed that three primers GLA-11, OPB-04 and OPD-02 showed good technical resolution and sufficient variations among different genotypes. A total of 40 RAPD bands were scored of which 38 (94.87%) polymorphic amplification products were obtained. Besides, the primer OPD-02 amplified maximum number of polymorphic bands (100.00%)...
April 2015
Isolation and identification of native lower fungi for polyunsaturated fatty acid (PUFA) production in Thailand, and the effect of carbon and nitrogen sources on growth and production
This research focuses on isolation and identification of polyunsaturated fatty acid (PUFA) producing fungi from natural sources of Thailand, followed by experimental designs for carbon and nitrogen utilizations of the potential strains. The results show that 8 and 16 isolates of lower fungi from freshwater and wet-fallen leaves, respectively, could produce PUFAs. Among these isolates, the NR06 that was isolated from...
April 2015
Molecular genetic diversity study of Lepidium sativum population from Ethiopia as revealed by inter simple sequence repeat (ISSR) markers
Lepidium sativum L. (family Brassicaceae), is an underutilized medicinal plant with worldwide distribution. In Ethiopia, L. sativum occurs in all regions and agro-ecologies at different altitudinal ranges. The study was conducted to assess the genetic diversity of L. sativum population from Ethiopia using inter simple sequence repeat (ISSR) marker. Molecular data generated from ISSR bands recorded was used for computing...
April 2015
Effects of selected culture media on mycellial growth of oyster mushroom (Pleurotus ostreatus)
The effect of selected culture media on mycelial growth of Pleurotus ostreatus was studied on six different culture media: three synthetic [malt extact agar (MEA), potato dextrose agar (PDA) and Saubroud dextrose agar (SDA)] and three formulated from locally available agricultural wastes [corncob (CCEA), palm waste (PWEA) and cassava peels (CPEA)]. The tissue culture of the mushroom was done on MEA which was used as the...
April 2015
The population structure of wild sorghum species in agro-ecological zones of Western Kenya
There is need to understand the genetic structure of wild sorghums that grow alongside cultivated traditional sorghum varieties in order to assess the potential effect of crop genes in wild populations. In this study, 175 wild sorghum samples were collected from 13 agroecological zones (AEZs) from three counties in Western Kenya and genotyped using microsattelite markers. Crop alleles were observed in wild sorghum...
April 2015
Activity of the fungus Pleurotus ostreatus and of its proteases on Panagrellus sp. larvae
Biological control has been shown to be one of the possible biotechnological applications of fungi and their proteases. The objective of this study was to evaluate the nematicidal activity of the fungus Pleurotus ostreatus and its proteases on Panagrellus sp. larvae. Proteolytic activity of P. ostreatus (PLO 06) was measured and characterized at different pHs, temperatures and in the presence of a inhibitor (PMSF)....
April 2015
Enhanced recombinant human blood coagulation factor VIII expression in the milk of goats using replication-defective adenovirus
High-level expression of recombinant human blood coagulation factor in milk of farm animals at a large scale provides a powerful tool for protein production. However, a bottleneck in recent protein synthesis technologies is the high cost of current transgenic livestock system. Here, we report a simple, rapid, and low-cost protein production method based on a replication-defective adenoviral vector system. The...
April 2015
Insights on predominant edible bamboo shoot proteins
Juvenile bamboo shoots have become a US$ 18 billion industry and have lured interest worldwide for its nutritive value and health enhancing properties; making it a suitable candidate for food security. Quantitative analysis revealed that juvenile bamboo shoots are a good source of proteins. However, there is no qualitative analysis describing unique proteins present in the edible bamboo shoots. In order to provide the...
April 2015
Effect of the somatic cell count on physicochemical components of milk from crossbred cows
The aim of the present work was to evaluate the effect of somatic cell count on the composition of milk in individual samples from crossbred cows at the Rio Verde municipality. Data from 2,730 individual samples of milk from crossbred cows were used. The samples were collected between February and April 2012 and analyzed at the Milk Quality Laboratory (Laboratório de Qualidade do Leite) in the Food Research...
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