April 2015
First detection of bla TEM, SHV and CTX-M among Gram negative bacilli exhibiting extended spectrum β-lactamase phenotype isolated at University Hospital Center, Yalgado Ouedraogo, Ouagadougou, Burkina Faso
Resistance to a wide variety of common antimicrobials is observed among clinical strains designed as extended spectrum β-lactmase (ESBL) producers. They produce enzymatic protein which inactivates efficiently oxyimino cephalosporin and constitutes a serious global health concern that has complicated treatment strategies. Many studies report high prevalence of ESBL producers among Gram negative bacilli. The aim of...
April 2015
Detection of Pseudomonas fluorescens from broth, water and infected tissues by loop-mediated isothermal amplification (LAMP) method
Loop mediated isothermal amplification is rapid, highly sensitive and specifically developed method for detection of bacterial infections. AprX gene for alkaline metalloprotease of Pseudomonas fluorescens was used to design four primers and loop mediated isothermal amplification (LAMP) conditions were standardized for amplification of DNA. LAMP primers successfully amplified P. fluorescens from DNA and bacterial cells...
April 2015
Detection of novel polymorphisms in the mitochondrial DNA D-Loop hypervariable region HVI from 400 healthy unrelated individuals from central and North-central Iraq
The mitochondrial DNA (mtDNA) is a small circular genome located within the mitochondria in the cytoplasm of the cell and a smaller 1.2 kilobase pair fragment, called the control region (D-loop). The aims of this research were to study this region by using the Sanger sequencing technique and establish the degree of variation characteristic of a fragment. FTA® Technology (FTA™ paper DNA extraction) was utilized...
April 2015
Oil palm fruit fibre promotes the yield and quality of Lentinus squarrosulus (Mont.) Singer, an edible Nigerian mushroom
Agricultural production and the agro-food industry furnish large volumes of solid wastes, which when unutilized could lead to environmental pollution. An attempt was made to utilize wastes from the oil palm and timber industries for the cultivation of Lentinus squarrosulus, a Nigerian edible mushroom. Mahogany sawdust (MSD), Gmelina sawdust (GSD), oil palm fruit fibre (OPFF) and oil palm empty fruit bunch (OPEFB)...
April 2015
Catalase activity of cassava (Manihot esculenta) plant under African cassava mosaic virus infection in Cape coast, Ghana
African cassava mosaic virus has caused an immersed low yield of the cassava crop. The virus impacts stress on the cellular metabolism of the plant producing a lot of reactive oxygen species and increases the expression of the antioxidant enzymes. The activity of catalase as a response to oxidative stress was investigated in this research. Cassava leaf extract infected with the African cassava mosaic virus and...
April 2015
Effect of collection time on the viability of banana pollen grains
The objective of this study was to assess the pollen viability of six improved diploid banana plants (AA) collected in different periods of the day, in two seasons of the year (winter and summer), using in vitro germination test and staining. Pollen grains collected at 8 am (anthesis), 10 am, 12 pm, 2 pm and 4 pm were evaluated. We used a culture medium for pollen germination containing 15% sucrose, 0.01% H3BO3, 0.01%...
April 2015
Bioprospecting of yeasts for amylase production in solid state fermentation and evaluation of the catalytic properties of enzymatic extracts
Profiling microorganisms with potential for amylase production in low cost culture media has been widely recognized due to its broad applicability. The aim of this study was to select yeast strains with potential to produce amylolytic enzymes by solid state fermentation. Fifty-four (54) strains were assessed and three exhibited ability to produce amylases: Candida parapsilosis with 14.68 U/mL (146.8 U/g substrate);...
April 2015
Antibacterial activity of watermelon (Citrullus lanatus) seed against selected microorganisms
This study was aimed at evaluating the effect of extraction methods on the antibacterial activity of Citrullus lanatus seed extract. C. lanatus (watermelon) is a popular fruit consumed all over the world. Three solvents were used for the extraction process: chloroform, methanol and distilled water while two extraction conditions- cold extraction and Soxhlet extraction (coded as hot in this study) were employed....
April 2015
Chemical characterization of passion fruit (Passiflora edulis f. flavicarpa) seeds
The aim of this study was to determine the chemical characteristics of passion fruit seeds and their oil for possible use in human food and reduction of organic waste from fruit industrialization. Passion fruit seeds were analyzed for moisture, lipids, proteins, ash, fibers, titratable acidity, pH, soluble solids and antioxidant activity. The oil was characterized for parameters such as acid value, saponification,...
April 2015
Mycosynthesis of iron nanoparticles by Alternaria alternata and its antibacterial activity
Nanotechnology is one of the most emerging fields in the recent years. In the current investigation, we report the biosynthesis of iron nanoparticles (Fe-NPs) employing Alternaria alternata fungus, which is an eco-friendly process for the synthesis of metallic nanoparticles. Fe-NPs were synthesized through the reduction of aqueous Fe3+ ion in the dark conditions. Ultraviolet–visible spectrum of the...
April 2015
Physicochemical parameters and antibiotics residuals in Algerian honey
The aim of the present study was to evaluate the quality of 36 samples of different honey type supplied by local producers from Algeria in order to verify its compliance with the standards of Codex Alimentarius and European Union (EU). For that, five physicochemical parameters were analyzed using the HPLC method: hydroxyl-methyl furfural (HMF), sugars, diastase activity and search of antibiotic contamination with...
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