African Journal of
Biotechnology

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12486

Table of Content: February 2020; 19(2)

February 2020

Isolation of ursolic acid from the leaves of Ocimum lamiifolium collected from Addis Ababa Area, Ethiopia

Ocimum lamiifolium is a well known medicinal plant in Ethiopia. Fresh leaves of the plant are squeezed and sniffed to treat colds and coughs and as an eye rinse for eye infections, while crushed leaves are used to arrest nose bleeding. In this study phytochemical investigation on the methanol extracts of this plant collected from Addis Ketema subcity, Addis Ababa. A triterpene, namely ursolic acid, was isolated using...

Author(s): Girma Birhanu  

February 2020

Morpho-molecular screening of wheat genotypes for heat tolerance

Wheat (Triticum aesum L.) production in Bangladesh is often impaired by heat stress. Therefore, it has been a priority to develop heat tolerant wheat variety for Bangladesh. An investigation was carried out to evaluate locally cultivated wheat genotypes for heat tolerance based on morpho-physiological and molecular markers. A pot experiment was carried out with ten locally cultivated wheat genotypes. Heat treatment was...

Author(s): Nihar Ranjan Saha, Md.Tariqul Islam, Md. Monirul Islam and Muhammad Shahidul Haque  

February 2020

Genetic diversity among Ethiopian sorghum [Sorghum bicolor (L.) Moench] gene bank accessions as revealed by SSR markers

The presence of genetic variation in plant populations is useful for conservation and use in breeding programs. This study was conducted to estimate the extent and patterns of genetic diversity among 200 sorghum accessions collected from different parts of Ethiopia and preserved in a gene bank.  Using 39 polymorphic simple sequence repeat (SSR) markers, which were previously mapped, 261 alleles were produced with...

Author(s): Alemu Tirfessa, Tesfaye Tesso, Asfaw Adugna, Hussein Mohammed and Dan Kiambi  

February 2020

Introduction of the exotic breeds and cross breeding of local chicken in Ethiopia and solution to genetic erosion: A review

This study reviews the introduction of the exotic breeds and cross breeding of local chicken in Ethiopia, solution to genetic erosion and needs for conservation with aim of delivering in the short form and clear information for beneficiaries and to whom it may concern. Poultry production and its product consumption are progressively increasing globally. In Ethiopia, chicken production plays a great role as a primary...

Author(s): Yonas Kejela  

February 2020

Effect of methanolic extract of Dennettia tripetala (pepper fruit) on biomarkers of oxidative stress and lipid peroxidation in type 2 diabetic male wistar rats

Oxidative stress resulting from chronic hyperglycemia in diabetes is due to high production of reactive oxygen species and/or a decrease in the antioxidant defense system activity. The study was designed to investigate the ameliorative effect of methanolic extract of Dennettia tripetala (DT) on oxidative stress and blood glucose level in type II diabetic male Wistar rats. Type II diabetes was induced by a single...

Author(s): Nwaeme Ogochukwu, Ude Victor Chibueze, Augustine Ndubuisi, Ugwudike Patrick, Agu Francis, Celestine Ani, Emeka Ugwuishi, Sunday Njoba, , Lilian Ndubuisi, , Hector Obianyido, Joachin Ezeokeke and Nwachukwu Daniel  

February 2020

Effectiveness of solid carriers on multiplication of bradyrhizobium mutant strain and nodulation of cowpea (Vigna unguiculata L)

Effective nodulation of legume is usually solid carriers dependent. Peat, the most effective solid carrier is not readily available in Nigeria, hence, alternative solid carriers were evaluated with Bradyrhizobium strains using cowpea as a test crop. Mutant strains (USDA 3384 and USDA 3451) were evaluated in the laboratory using solid carriers and pot experiments. The survival (multiplication), infectivity and nodulating...

Author(s): Nwokocha, A. G., Fagbola, O. and Daramola, D. S.  

February 2020

Chemical and physico-chemical characterization of seeds of melon cultivars: Valenciano and Pele de Sapo, cultivated in the Amazon

The aim of this work was to perform chemical and physical-chemical analyzes of the oil of the cultivars Valenciano Amarelo melon (VA) and Pele de Sapo melon (PS) (Cucumis melo L.) produced in Boa Vista, Roraima, Brazil, by GC -FID, IR, 1H NMR and the thermogravimetric behavior TG/DTG as well as yield of that obtained oil. Thus, the oil yield of Valenciano melon seeds was 28.47% and that of Pele de Sapo melon was 26.41%....

Author(s): Flavio Paulino da Silva, Ismael Montero Fernández, Selvin Antonio Saravia Maldonado, Simone Rorigues da Silva, Ricardo Santos Alemán, Vany Perpetua Ferraz, Jhunior Abrahan Marcia Fuentes and Bernardo de Morais Linhares  

February 2020

Analytical quantification of copper in frogs (Rana tigrina) found from various aquatic habitats

This study focused on the quantitative determination of Cu metal in various physical parts of frogs by using the Atomic Absorption Spectrometry technique). Cu was measured (2.72±0.77 µg/g) in kidney of Rana tigrina captured from industrial site and in R. tigrina captured from non-industrial site (28.10±27.02 µg/g). Similarly, 27.56±9.92 µg/g Cu was discovered in liver of R. tigrina...

Author(s): Muhammad Nasir, Tariq Mahmood Ansari, Hira Javed, Ghulam Yasin, Aleem Ahmad Khan and Muhammad Shoaib  

February 2020

Immobilization of the Carioca bean (Phaseolus vulgaris L.) extract on SiO2-TiO2 aiming the determination of the herbicide Simazine

Pesticides are identified as hazardous contaminants due to high toxicity and persistence in the environment. In this context, it is necessary to monitor these pollutants in the environment in situ, with the use of low cost analytical techniques and of quick response. Biosensors are presented as a complementary analytical tool to more complex techniques, such as chromatography. The present work aims to develop, in a...

Author(s): Francisca das Chagas Sobral Silva Mihos, Emerson Schwingel Ribeiro, Maria Helena Wohlers Morelli Cardoso, Lucia Helena Pinto Bastos, Lívia Maria da Costa Silva and Andréa Medeiros Salgado