May 2004
High frequency plant regeneration from desiccated calli of indica rice (Oryza Sativa l.)
An efficient and reproducible protocol is required to achieve high frequency transformation from transformed calli. We report here high frequency plant regeneration from mature seed derived embryogenic calli of two recalcitrant indica rice cultivars HKR-46 and HKR-126 after partial desiccation treatment. Embryogenic and nodular callus was initiated on MS basal medium supplemented with 2.5 mg l-1 2,4-D,...
May 2004
Improving bambara groundnut productivity using gamma irradiation and in vitro techniques
In recent times efforts are being made to improve the productivity of bambara groundnut. Studies were initiated (i) to characterise and evaluate landraces and to select superior ones for irradiation, (ii) to induce genetic variation through gamma irradiation and (iii) to use biotechnological approaches to shorten the generation cycle. The results of the study indicated that gamma irradiation induced higher genetic...
May 2004
Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. I. The role of colony morphology and nitrogen sources in productivity
A systematic approach to process optimization for production of rifamycin B was applied to a strain of Amycolatopsis mediterranei. Examination of the growth revealed 6 different morphologically distinct colonies on Bennett’s agar medium. Rifamycin B production in shake flasks by the six different colony types ranged between 0.5 and 1.2 g/l. There was a clear correlation between the colony morphology and...
May 2004
Optimization of industrial production of rifamycin B by Amycolatopsis mediterranei. II. The role of gene amplification and physiological factors in productivity in shake flasks
Amplification of gene expression of the most productive colony type of Amycolatopsis mediterranei strain N1 under stress of chloramphenicol, resulted in isolation of a variant NCH with productivity of 2.56 g/l compared to 1.15 g/l by the parent strain N1 (2.2 fold increase) . This amplified variant has a further advantage of reduced variation in colony morphology with predominance of the most productive colony...
May 2004
Discrimination of Bacillus sphaericus strains by filtrate protein profiles
A total of 19 strains of Bacillus sphaericus are compared both in vegetative and sporulated stages according to their filtrate protein profiles obtained by Native-PAGE and SDS-PAGE. When the strains are compared in the sporulated stage, filtrate protein profiles obtained by Native-PAGE differentiated the strains according to their phage and serogroups. On the other hand, the typing according to...
May 2004
Evaluation of the aqueous extract of Boswellia dalzielii stem bark for antimicrobial activities and gastrointestinal effects
The aqueous extract of Boswelli dalzielii Hutch (family: Burseraceae) was investigated for therapeutic properties using aspirin-induced ulceration in rats, gastrointestinal motility in mice and castor oil-induced diarrhoea in rats. The median lethal dose (LD50) of the extract was carried out via the oral route in mice. Antimicrobial and preliminary phytochemical screening of the extract was also investigated....
May 2004
Recombinant Poliovirus circulation among healthy children immunized with oral polio vaccine in Abidjan
In order to assess the level of polio virus with natural recombinant genome and wild polio virus circulating in the environment of healthy children aged 0 to 5 years in Abidjan, 130 polio viruses made up of 26 polio type 1, 55 type 2 and 49 type 3 were identified by neutralisation test with monoclonal antibodies and restriction fragment length polymorphism (RFLP) targeting the VP1 and 3D1 gene. Four wild non...
May 2004
Histological exploration of follicular population of the Moroccan bovine (Oulmes-Zaers) breed
Follicular population and repartition in the ovarian cortex was investigated in the ovaries of the Oulmès-Zaers cattle breed and its crosses. A total of 30 ovaries were collected at slaughterhouse in Casablanca and Rabat from Oulmes-Zaers breed and its crosses (2 genotypes) of 3 age groups (<3; 3 to 5; >5 years). The histological study of the ovary revealed that the ovarian cortex is composed of different...
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