African Journal of

  • Abbreviation: Afr. J. Biotechnol.
  • Language: English
  • ISSN: 1684-5315
  • DOI: 10.5897/AJB
  • Start Year: 2002
  • Published Articles: 12487

Table of Content: October 2019; 18(30)

October 2019

Selection of Lactobacillus strains newly isolated from Algerian camel and mare fermented milk for their in vitro probiotic and lipolytic potentials

The main objective of this study was the characterization of new lactobacilli probiotic strains belonging to lactic acid bacteria (LAB). Eighty-eight strains were isolated from different Algerian camel and mare fermented milks; three of them were pre-selected for their stability, fast growth and resistance to acidity and bile salts. Cell viability was assessed in simulated gastric and intestinal conditions. On the other...

Author(s): Sabrina Amara, Halima Zadi-Karam and Nour-Eddine Karam

October 2019

Optimizing DNA isolation protocol for rosemary (Rosemarinus officinalis L) accessions

Rosemary plant is in high demand due to its application in traditional health care, food flavoring, fragrance and pharmaceutical industries. It contains high level of secondary metabolites which are responsible for its beneficial activities. Application of molecular techniques would facilitate the production of these substances and screening of accessions. The isolation of polymerase chain reaction (PCR) amplifiable...

Author(s): Zewdinesh Damtew Zigene, Bizuayehu Tesfaye Asfaw and Tesfaye Disasa Bitima  

October 2019

Nutritional enhancement of cocoa pod husk meal through fermentation using Rhizopus stolonifer

This study evaluated the effect of fermentation period on the proximate composition, anti-nutritional content, fibre fractions and amino acid profile of cocoa pod husk meal (CPHM). Cocoa pod husk was taken through a solid state fermentation process involving Rhizopus stolonifer as its starter culture for a period of two weeks. The fermented CPHM was dried and analyzed for its proximate composition, anti-nutritional...

Author(s): Olugosi O. A., Agbede J. O., Adebayo I. A., Onibi G. E. and Ayeni O. A.  

October 2019

Variable DNA methylation in Ensete (Ensete ventricosum) clones associated with developmental stage revealed by Amplified Fragment Length Polymorphisms (AFLPs) with Methylation-Sensitive enzyme

Ensete (Ensete ventricosum) is an important perennial species in Ethiopia. It is used for food and fiber. The mode of propagation is asexual and sexual. Asexual form of reproduction is by natural and anthropogenic induced sucker. This research aims to investigate and measure the methylation diversity of natural and anthropogenic induced sucker forming Ensete. Twenty-seven individuals from Hawassa University research...

Author(s): Hewan Demissie Degu and Bizuayehu Tesfaye  

October 2019

Development and validation of an analytical method for quantification of total flavonoids in Alternanthera brasiliana by ultraviolet-visible absorption spectrophotometry

Alternanthera brasiliana is popularly known in Brazil as "penicillin" or "benzetacil" and is used in traditional medicine for the treatment of infections and for the healing of wounds. It is also used as an ornamental plant due to the characteristic purple coloration of its leaves when cultivated in shade. The objective of this study was to develop and validate an analytical methodology by...

Author(s): José M. T. de Alencar Filho, Hyany A. P. Teixeira, Iure S. de Carvalho, Milenna V. V. de O. Alencar, Nathália A. C. de Souza, Tarcísio C. de L. Arraújo, Emanuella C. V. Pereira, Isabela A. Amariz, Pedrita A. Sampaio, Pedro J. Rolim-Neto, Larissa A. Rolim, and Edigênia C. da C. Araújo,  

October 2019

Characterization of three full Iris yellow spot virus genes of a garlic-infecting isolate from Zimbabwe using next-generation sequencing technology

Iris yellow spot virus (IYSV) is an important pathogen of Allium species worldwide. It has a tripartite genome consisting of the large (L), medium (M) and small (S) RNA segments. Despite its worldwide distribution, very few complete gene and genome sequences are available in public databases. The aim of this study was to obtain full gene sequences of a garlic-infecting IYSV isolate by next-generation sequencing (NGS)...

Author(s): Charles Karavina, Jacques Davy Ibaba and Augustine Gubba  

October 2019

Economic viability of the biogas produced on pig farms in Brazil for electric power generation

Electric energy generated by clean and renewable sources, such as biogas, is a subject widely discussed and of global importance. Biogas, besides being an alternative to other fuels uses a raw material, which in many cases is considered disposable, worthless and harmful to the environment when not correctly disposed of. Several factors influence the design, operation and collection of the final product. This paper is a...

Author(s): Levi Mariano Neto, Wellington Henrique Ponciano and Antonio Manoel Batista da Silva

October 2019

Scientific applications and prospects of nanomaterials: A multidisciplinary review

Nanotechnology is the science of objects <100 nm in size. Research into the development and application of nanomaterials takes a material science and engineering-based approach to nanotechnology. Nanomaterials often possess interesting optical, electronic and mechanical properties. The capacity to construct large, intricate structures with nanometer precision is rapidly increasing and consists several top-down...

Author(s): ADEOLA Adedapo Oluwasanu, FAPOHUNDA Oluwaseun, JIMOH Adeleke Teslim, TOLUWALOJU Tunde Isaiah, IGE Ayokunle Olalekan and OGUNYELE Abimbola Chris  

October 2019

Comparison of embryo developmental rates in Nguni, Bonsmara and Boran beef cattle breeds following in vitro fertilization and artificial insemination

The aim of this study was to compare the embryonic developmental rates in the Southern African cattle breeds. To do this, cryopreserved semen straws from Nguni, Bonsmara, and Boran bulls were thawed at 38°C and evaluated for sperm motility characteristics using Sperm Class Analyser (SCA). The fertilizing ability of frozen/thawed sperm was evaluated by performing artificial insemination (AI) and in vitro...

Author(s): Mohleko Helen Mapeka, Cyril Mpho Pilane, Robert. Treadwell, Jones Ng’ambi and Cuthbert Banga  

October 2019

Genetic diversity assessment of yams (Dioscorea spp.) from Ethiopia using inter simple sequence repeat (ISSR) markers

The genetic diversity and relationships of 70 accessions of yam belonging to Dioscorea cayenensis/Dioscorea rotundata complex (55), Dioscorea bulbifera (13) and Dioscorea alata (2, as a reference) were assessed using six inter simple sequence repeat (ISSR) primers. DNA was extracted from a bulk of two plants per accession using a modified cetyl trimethyl ammonium bromide (CTAB) method. Six ISSR primers amplified 77...

Author(s): Kedra Mohammed Ousmael, Kassahun Tesfaye, and Teklehaimanot Hailesilassie