African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Isolation, identification and characterization of a straw degrading Streptomyces griseorubens JSD-1

H. W. Feng1,3, Y. E. Zhi1,3, W. W. Shi2,3, L. Mao1,3 and P. Zhou1,2,3*
1School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.  2School of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, China.  3Key Laboratory of Urban Agriculture (South) of Ministry of Agriculture, Shanghai 200240, China. 
Email: [email protected]

  •  Accepted: 25 March 2013
  •  Published: 28 May 2013

Abstract

To reutilize rice straw immediately during the agricultural production process, an actinomycete named 

JSD-1 was isolated from soil and rotten straw using multiple selective culture media. It was identified as 
Streptomyces griseorubens through polyphasic taxonomy consisting of morphological and 
physiological characterization combined with the result of 16S rDNA gene sequence and phylogenetic 
analysis. It also released a series of enzymes, including cellulase, pectinase, xylanase and ligninase 
simultaneously. According to the experiment of weight loss, JSD-1 decomposed about 76% of the straw 
in 30 days. Besides, it had the ability to resist certain plant diseases, such as Sclerotinia rot of melon 
and so on. It was obviously that JSD-1 could degrade rice straw effectively and had great potential of 
being applied to the utilization of straw resource. 
 
Key words: Rice straw, Utilization, Streptomyces griseorubens, Polyphasic taxonomy, enzymes, weight loss, 
diseases.