May 2013
A review of pentose utilizing bacteria from bagasse hemicellulose
There has been considerable increase in interest in the conversion of renewable resources into several essential products (for example, biofuel, amino acids, among others) over the last few years, as the potential impact of global warming is demanding technologies that close the carbon cycle. Utilization of the hemicellulose fraction from lignocellulosic biomass is an important factor to optimize for the economicallyl...
May 2013
Probiotics use in intensive fish farming
The use of probiotics in feed for fish and its inclusion in intensive aquaculture to promote healthy gut is growing. The need for alternative measures that will perform closely and effectively to the use of antibiotics after its annulment in European Union (EU) in 2006 is an issue which call for concern. Several definitions of probiotics mainly for aquaculture were also dig out from published papers. Among them is a...
May 2013
Optimization of factors affecting protoplast preparation and transformation of smoke-tree wilt fungus Verticillium dahliae
Verticillium dahliae is a causal agent of vascular wilt smoke-tree Cotinus coggygrial. The genetic transformation of V. dahliae has been carried out by Agrobacterium and polyethylene glycol-mediated transformation. Due to technical simplicity and efficiency, PEG-mediated transformation is the most commonly used method for genetic transformation in filamentous fungi. However, polyethylene...
May 2013
Optimizing antimicrobial activity of the bovine lactoperoxidase system against Salmonella enterica Hadar, a causative agent of human gastroenteritis in Tunisia
In this work, it was shown that activating the Lactoperoxidase system (LP system) has an antimicrobial effect against Salmonella enterica serovar Hadar (S. enterica Hadar), a foodborne pathogen widespread in Tunisia and therefore a major cause of gastroenteritis. The aim here was to optimize the concentrations of the various compounds used in activating the LP system and to test the efficiency of...
May 2013
Effect of antimicrobial activity of sodium hypochlorite and organic acids on various foodborne pathogens in Korean ginseng root
Ginseng is a widely used therapeutic medicinal agent for a number of diseases. It is generally distributed in both processed and unprocessed whole roots and is the most liable to be contaminated by microorganisms as harvest progresses and during manufacturing process. Thus, decontamination process is necessary for combating the microbiological quality of the products. Here, we examined the effect of sodium hypochlorite...
May 2013
Isolation, identification and characterization of a straw degrading Streptomyces griseorubens JSD-1
To reutilize rice straw immediately during the agricultural production process, an actinomycete named JSD-1 was isolated from soil and rotten straw using multiple selective culture media. It was identified as Streptomyces griseorubens through polyphasic taxonomy consisting of morphological and physiological characterization combined with the result of 16S rDNA gene sequence and...
May 2013
Thermal inactivation of Alicyclobacillus acidoterrestris spores isolated from a fruit processing plant and grape juice concentrate in South Africa
Thermal inactivation at 95°C for two strains of Alicyclobacillus acidoterrestris isolated from contaminated fruit juice concentrates were investigated in a 0.1% (m/v) peptone buffer solution (pH 7.04) and grape juice (pH 4.02, 15.5 °Brix). The thermal inactivation ofA. acidoterrestris spores followed first-order kinetics, suggesting that as the microbial population is exposed to a...
May 2013
Intestinal infections in malnourished children in south of Tehran, Iran
Intestinal and urinary tract infections are the most common infections in malnourishedchildren. In the present study, intestinal infections were surveyed in malnourished children.The present research was a cross sectional study in 12 months. During the study period, all the malnourished children admitted to health center in south of Tehran, were...
May 2013
Potential of colloidal or silver nanoparticles to reduce the growth of B16F10 melanoma tumors
Previosuly, we reported the cytotoxic effect of colloidal silver (AgC) on MCF-7 breast cancer cell line. However, there is scarce information on its antitumor potential. The aim of this study was to evaluate the anti-tumoral activity of colloidal silver (AgC) or silver nanoparticles (AgNPs) in a B16F10 melanoma mice model. In vitro, B16F10 cells were treated with different concentrations of AgC or AgNPs and cell...
May 2013
Motal cheese of Iranian nomadic tribes as an untouched source of potentially probiotic Lactobacilli
Several studies have revealed the positive impacts of probiotic microorganisms on human health and physiology. Since the diversity of human microbiota is influenced by factors such as lifestyle and nutrition, looking for appropriate supplies to maintain the balance of gastrointestinal flora should be considered as high priority. In the present study, Motalcheese, a special kind of traditional cheese made inside...
May 2013
Response surface analyses of rhamnolipid production by Pseudomonas aeruginosa strain with two response values
Response surface methodology (RSM) and Box-Behnken experiment design (BBD) were employed for optimization of biosurfactant shaking flask fermentation conditions of a Pseudomonas aeruginosa strain. Using Design Expert 8.0.5b software, multiple binomial mathematical models were established to study the influence of carbon sources (waste vegetable oil, WVO), nitrogen source (NaNO3) of and potassium source (KCl) levels in...
May 2013
Effect of pesticides on available cationic micronutrients along with viable bacteria and fungi in soil
A pot culture experiment was conducted to investigate the effect of pesticides – 2,4-D (herbicide), endosulfan (insecticide) and dithane M-45 (fungicide) at their field application rates (1.0, 2.0 and 1.5 kg a.i ha-1), respectively, on the available Diethyl Triamine Pentacetic acid (DTPA) extractable copper (Cu), manganese (Mn) and zinc (Zn) along with the population of viable bacteria and fungi in new alluvial...
May 2013
Biological treatment for enhancement of the antioxidant capacity in agro-biomass as animal feed
Rice straw was fermented by two strains of Aspergillus terreus (ATCC 74135 and ATCC 20542) to enhance the antioxidant content of this agro-biomass as ruminant feed. After 8 days of fermentation, antioxidant capacity of samples was determined using Ferric reducing antioxidant power (FRAP), 2,2′-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 1,1-diphenyl-2-picryl- hydrazyl ...
May 2013
Mobile phones of health care workers are potential vectors of nosocomial agents
Mobile phones have become necessary companions to most health care personnel and have been implicated as reservoirs of known nosocomial agents. This study was carried out to determine the type and frequency of microorganisms’ contaminating mobile phones of health workers in a University Teaching Hospital in Nigeria. Swabs of 50 mobile phones were collected and cultured, and contaminants characterized. Forty three...
May 2013
Identification and antimicrobial susceptibilities of 21 strains of Salmonella from poultry
A total of 21 Salmonella strains were isolated from clinical cases in Yangzhou, China, and the surrounding areas from Feb 2011 to Dec 2011. All isolates were screened by PCR amplification for InvA gene, which is specific to the Salmonella genus. Thus, twenty-oneSalmonella were identified from the specimens. The isolates belonged to 6 serovars ofSalmonella. Both Salmonella...
May 2013
Isolation and characterization of bacteriophages against enterotoxigenic Escherichia coli strains causing swine diseases
Six lytic bacteriophages (JH1-JH6) lytic against O8 ETEC were isolated from 40 individual fecal samples and 30 sewage samples from pig farms in Nanjing, China. Two of the bacteriophages (JH1 and JH2) with icosahedral heads, necks, contractile tails belonged to the Myoviridae family. The other four bacteriophages (JH3, JH4, JH5 and JH6) had relatively thin, long, non-contractile and belonged to...
May 2013
Isolation, identification and alkane hydroxylase genes detection of a marine diesel-degrading bacterial strain (F9)
A diesel-degrading bacterial strain (F9) was isolated and identified in this study. F9 strain was isolated from diesel polluted seawater. It was a Gram-negative, non-spore-forming bacterium and identified as a strain of Acinetobacter sp. according to its physiological and biochemical properties and 16S rDNA sequence analysis. It had efficient diesel-biodegradability and could grow very well with diesel as...
May 2013
Actinobacillus pleuropneumoniae secretes a metalloprotease that degrades porcine fibrinogen
Fibrinogen is an important molecule in homeostasis and coagulation, besides its interaction with different host cells and participation in inflammatory process. Bacteria possessing fibrinogen-binding proteins can evade the immune host response by covering those molecules. Fibrinogen degradation can induce intravascular coagulation and depletion of fibrinogen produces bleeding in the affected...
May 2013
Microbiological properties of Turkish Beyaz cheese samples produced with different probiotic cultures
In this study, Turkish Beyaz cheese samples were produced by adding commercial lactic culture [Lactococcus lactis and Lactococcus cremoris (A)] and different probiotics[Bifidobacterium bifidum BB-12 (B), Bifidobacterium bifidum BB-12+Lactobacillus acidophilus LA-5 (C), Bifidobacterium bifidum (D) and Bifidobacterium longum (E)]. Probiotic cultures were added to cheese milk at least 107 cfu/ml levels....
May 2013
An efficient method to produce recombinant bacterial effector Tir coupled cytoskeleton protein (TCCP) complexed with host protein insulin receptor tyrosine kinase substrate (IRTKS)
An important pathogenic process in enterohemorrhagic Escherichia coli (EHEC)associated diseases is the formation of attaching and effacing (A/E) lesions, which are a typical pathological change in host cells. The classical pathway for A/E lesion formation requires the participation of proteins from both bacteria and host cells, namely intimin, translocated intimin receptor (Tir), insulin receptor tyrosine...
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