African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Evaluation of phenol degradation by effective microorganism (EM) technology with EM-1

S. Sivasubramanian1 and S. Karthick Raja Namasivayam2*
1Department of Biotechnology, Sathyabama University, Chennai, Tamil Nadu, India. 2Department of Chemical Engineering, Sathyabama University, Chennai, Tamil Nadu, India.
Email: [email protected]

  •  Accepted: 16 July 2013
  •  Published: 09 August 2013


Effective microorganisms (EM) are a culture of co-existing beneficial micro-organisms predominantly consisting of lactic acid bacteria, photosynthetic bacteria, yeast, fermenting fungi and actinomycetes that are claimed to enhance the decomposition of organic matter which in turn improves soil fertility and toxic pollutants including petroleum hydrocarbons, among others. In the present study, EM-1 commercial formulation was evaluated for phenol degradation under batch condition with 250, 500, 750 and 1000 mg/l of synthetic phenol. The biomass concentration, residual phenol concentration, speficic growth rate and specific degradation rate was recorded at respective concentration under different time interval. EM-1 could tolerate all the tested concentration of phenol and the maximum specific growth rate and degradation rate was recorded at 250 mg/l of phenol supplemented media followed by 500, 750  and 1000 mg/l.


Key words: Effective microorganism, EM-1, phenol degradation.