Abstract

Viruses are major limitations to cultivation. These viruses were detected by double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using specific polyclonal antibodies for Banana bunchy top virus (BBTV) and Cauliflower mosaic virus (CMV). Polymerase chain reaction, (PCR) based detection of a 500 bp amplicon from BBTV infected tissues and or a 600 bp amplicon from infection Brome mosaic virus (BMV) infected tissues confirmed the presence of the viruses in these plants. As well as the major deoxyribonucleic acid (DNA) fragments of expected size, 500 bp was amplified from BBTV infected tissues and the size of the major amplified product in BMV infected tissues was 600 bp. The application of banana meristem tip (0.3 mm) is more effective for BBTV and or BMV eradication in vitro. Chitosan (0.12%), treatment for infected plants was more effective for BBTV and BMV eradication in vivo. The results proved that there is five important precautions for success of the rouging program of banana viral control included: (1) To ensure that the nursery stock is clean and free from latent virus infection via starting tissue culture seedlings virus tested or suckers treated with 0.12% chitosan, (2) Detecting infected plants periodically every month by fortnightly inspection via external symptoms and every season by a DAS-ELISA test for the presence viral diseases (3) Rouging the infected plants after two inspections. The rouged plants were destroyed by burning at the end of growing season, (4) Spraying the plants and weeds with malathion and cilecron every two weeks alternatively to kill the aphid vectors from the first April to end of growing season is December, (5) Eradication of woods and grasses from plantations (secondary virus hosts) by digging up and inherbicide. Dealing with this problem as a community.

Key words:    Banana, nursery, orchard, banana bunchy top virus (BBTV), Brome mosaic virus (BMV) in vitro, in vivo, eradication, PCR, ELISA.