African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Preparation of scFv against HrpA of Pseudomonas syringae pv. tomato DC3000

Yanling Yang, Hairong Liu, Ziqin Zheng, Rongzhi Wang, Shihua Wang and Zhenhong Zhuang*
Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
Email: [email protected]

  •  Accepted: 08 October 2013
  •  Published: 07 November 2013


Hrp pilus is a pathogenic bacterial surface appendage which serves as a tool for the transformation of bacterial effecter proteins into the plant cell. Hrp pilus is assembled by HrpA protein. In this study, a HrpA protein was successfully expressed, purified and a phage library single-chain fragment variable (scFv ) was constructed. After six rounds of bio-panning, a specific scFv against HrpA protein named scFv-FE5 was screened by phage display. The results of enzyme-linked immunosorbent assay (ELISA) and western blotting analysis showed that scFv-FE5 could recognize HrpA protein specifically. Current work demonstrates the preparation of the antibody anti-HrpA scFv-FE5 using phage display technology, a very useful tool for generation of a specific scFv to a certain antigen.


Key words: HrpA, expression, scFv, phage display, bio-panning.