November 2013
Identification of Salmonella isolated from pork sausage and evaluation of thermal and antimicrobial resistance of isolates
Pork products are important reservoir of Salmonella. In Brazil, the consumption of pork sausage is high and the occurrence of Salmonella in product is usual. Salmonella occurrence was studied in twenty samples of fresh pork sausage. Pulsed-Field Gel Electrophoresis (PFGE) was performed to characterize Salmonella isolates. Their antimicrobial and...
November 2013
Effect of lipopolysaccharide on nitric oxide (NO) production in culture supernatants exposed to Bacillus Calmette-Guerin (BCG) and supplemented with 1, 25(OH)2D3
Recently, there has been a striking increase in the number of patients with mycobacterial disease, and this is an important health problem around the world. One essential component of tubercular host defense includes nitric oxide production. The objective of this study was to investigate in vitro the effect of lipopolysaccharide on nitric oxide production in supernatants of mononuclear cell cultures from Mycobacterium...
November 2013
Isolation of salt tolerant endophytic and rhizospheric bacteria by natural selection and screening for promising plant growth-promoting rhizobacteria (PGPR) and growth vigour in tomato under sodic environment
The importance of plant growth promoting rhizobacteria in growth promotion and their ability to elicit ‘induced systemic tolerance’ against abiotic stresses has been documented. However, the performance of these microbes under various abiotic stresses especially saline-sodic conditions will be of great importance in the current agricultural scenario. In this study, we isolated 16 rhizobacteria through...
November 2013
Preparation of scFv against HrpA of Pseudomonas syringae pv. tomato DC3000
Hrp pilus is a pathogenic bacterial surface appendage which serves as a tool for the transformation of bacterial effecter proteins into the plant cell. Hrp pilus is assembled by HrpA protein. In this study, a HrpA protein was successfully expressed, purified and a phage library single-chain fragment variable (scFv ) was constructed. After six rounds of bio-panning, a specific scFv against HrpA protein named scFv-FE5 was...
November 2013
Antibacterial activity of leaves extracts of Jatropha curcas and Euphorbia heterophylla
The antibacterial activity of aqueous and ethanolic extracts of two Nigerian medicinal plants, Euphorbia heterophylla and Jatropha curcas was investigated using disc agar diffusion and broth dilution assays against four clinical isolates of bacteria consisting of two gram positive (Staphylococcus aureus and Enterococcus faecalis) and two gram negative bacteria (Escherichia coli and Pseudomonas aeruginosa). The...
November 2013
Molecular epidemiology and vaccine matching study on foot-and-mouth disease virus circulating in Ethiopia
The study was conducted in three regional states of Ethiopia: Amhara, Oromia, and Addis Ababa with the aims of identifying the molecular epidemiology of Foot and Mouth Disease (FMD) virus in Ethiopia and to determine the appropriate vaccine strains of FMD virus. From the total of 33 bovine epithelial tissue cultured samples, 19 (57.57%) samples were showed cytopatic effect for FMD virus. Of these, three samples were...
November 2013
Diagnostic assessment of Xpert MTB/RIF in a sample of Mycobacterium tuberculosis Egyptian patients
Global Mycobacterium tuberculosis (MTB) control efforts have been severely hampered by the lack of diagnostic tests that are accurate, simple to use and can be applied at the point of clinical care. This compounded by the widespread inability to test for drug resistance. The newly developed Xpert MTB/RIF assay utilizes real-time PCR technology to both diagnose TB and detect rifampicin (RIF) resistance concurrently using...
November 2013
Antimicrobial activity of leaf and fruit extracts of Jordanian Rubus sanguineus Friv. (Rosaceae)
In vitro antimicrobial activity of ethanolic and methanolic extracts of the leaf and fruit of Rubus sanguineus were investigated against pathogenic strains of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus cereus and Candida albicans using agar well diffusion and microdilution broth assays. This study showed that leaf ethanolic extract exhibited the best antimicrobial activity with zone of...
November 2013
Isolation and characterization of a Bacillus strain for alkaline wastewater treatment
Biological treatment is one of the considerable choices for removing of organic pollutants present in petrochemical wastewaters. In this study, five strains, named as BS1, BS2, BS3, BS4 and BS5, were isolated from sludge close to a petroleum smelter. BS5, the isolate with the highest chemical oxygen demand (COD) removal rate, was identified as Bacillus flexus, based on 16S rDNA sequences. Subsequently, the optimized COD...
November 2013
Seasonal variation of citrinin in traditionally brewed African beer
A seasonal study on citrinin concentrations in traditional beer was conducted using a standardised enzyme-linked immunosorbent assay. Representative samples of indigenous South African beer were collected during the hot, mild and cold seasons from marginal-urban settlements in the Kimberley area, South Africa. The mean temperature and humidity readings were 37°C and 29% for respectively summer, 19.4°C and 7.1%...
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