African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5230

Full Length Research Paper

Cytokine genes expression in mice hepatocytes during malaria infection

Saad Alkahtani1*, AL-Farraj S. A.1, Saud A. Alarifi2, AL-Eissa Mohammed saad3 and Al-Dahmash B4
1Department of Biology, Teachers College, King Saud University, Riyadh, Saudi Arabia. 2Department of Zoology, Science College, King Saud University, Riyadh, Saudi Arabia. 3Department of Biology, Science College, Hail University, Hail, Saudi Arabia. 4Department of Medical Laboratory, College of Health Sciences, King Saud University, Riyadh, Saudi Arabia.
Email: [email protected]

  •  Accepted: 30 July 2011
  •  Published: 18 August 2011

Abstract

The aim of this study was to investigate the inflammation genes (IL-1β, IL-6, TNF-α and iNos) responses to Plasmodium chabaudi malaria in the liver of female C57BL/6 hepatocytes via mRNA expression due to infection with P. chabaudi at different time points. Mice were injected intraperitoneally (ip) with 106 P. chabaudi-infected erythrocytes and then scarified at days (0, 1, 4 and 8 respectively). RT-PCR was used to quantify liver inflammation genes. The levels of IL-1β, IL-6 and TNFα were significantly increased at days 1 and 8. The total iNos were significantly increased at all days after infection. In conclusion, present data has shown that infection with P. chabaudi stimulated infalammation genes in the liver. Thus, we suggest the implication of oxidative stress due to outcome of malaria in mice hepatocytes according to its natural function need to be confirmed with a larger number of samples to be used as a reliable inflammation detection method.

 

Key words: Inflammation, malaria, mice, liver.