African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Table of Content: 18 August, 2011; 5(16)

July 2011

Isolation and characterization of a psychrophilic bacterium producing cold active lactose hydrolyzing enzyme from soil of Mt. Himalaya in Nepal

Psychrophilic bacteria, which grew on lactose as a carbon source, were isolated from soil of Island Peak area at Mt. Himalaya in Nepal, and identified. 148 bacterial isolates were isolated via X-gal hydrolysis on plate. Among these, three isolates, referred to as KNOUC 401, 402 and 403, displayed good cold-adapted growth and activity for hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) and...

Author(s): E. S. Nam, Y. H. Kim, K. H. Shon, and J. K. Ahn

August 2011

Nigerian phages: The first bacteriophages from Tropical Africa

Fifteen morphological bacteriophage types were observed in enrichment cultures of sewage, surface water and human feces collected in Lagos, Nigeria. All phages were tailed and belonged to the Myoviridae, Siphoviridae or Podoviridae families. Most phages could be assigned to well-known morphotypes. Ten of them corresponded to phages of enterobacteria or pseudomonads. Four morphotypes (fKZ, T4, c,...

Author(s): Adeniran S. Koko, Hans W. Ackermann, Monisola A. Taiwo and Sunday A. Omilabu

August 2011

Evaluation of real-time PCR for Mycobacterium ulcerans in endemic region in Côte d’Ivoire

Buruli ulcer (BU) is caused by a mycobacterium called Mycobacterium ulcerans. The events of BU are the skin lesions. The lack of early diagnosis and treatment cause severe disability. Today the emergence to BU in Africa and particularly in Côte d’Ivoire needs faster diagnosis to control and to prevent the infection by M. ulcerans. The surveillance of BU is difficult, because the transmission...

Author(s): Elise Solange Ngazoa-Kakou, Euloge Ekaza, Nguetta Aka, David Coulibaly-N’Golo, Bakary Coulibaly and Mireille Dosso,

August 2011

Contamination of cattle carcasses by Escherichia coli shiga like toxin with high antimicrobials resistence

During processing of cattle carcasses, contamination may occurs with the transfer of microbiota of animals feaces to carcasses. This contamination many times may be byEscherichia coli carriers of virulence factor as stx and eae genes being classified as Shiga like toxin. Shiga toxin-producing Escherichia coli (STEC) is recognized wordwide as human pathogen. A survey was performed to...

Author(s): Everlon Cid Rigobelo, Renato Pariz Maluta, Clarissa Araújo Borges, Lívia Gerbasi Beraldo, Manoel Victor Franco, Lemos Sirlei Aparecida Maestá and Fernando Antonio de Ávila

August 2011

Determination of insecticidal toxicity of three species of entomopathogenic spore-forming bacterial isolates against Tenebrio molitor L. (Coleoptera: Tenebrionidae)

Bioassays were conducted using larvae of mealworms, Tenebrio molitor, to determine lethal concentration for five entomopathogenic strains of spore-forming bacteria. Lethal concentration was determined by feeding T. molitor larvae cabbage discs dipped in whole cell cultures of these five strains of bacteria. The strains of bacteria were isolates ofBacillus cereus, Bacillus...

Author(s): N. Du Rand and M. D. Laing

August 2011

What changed in necrotizing fasciitis in twenty-five years?

Necrotizing fasciitis (NF) is a deadly soft tissue infection characterized by necrosis of subcutaneous tissues. In this study, our aim was to identify variables affecting patient outcome and mortality in necrotizing fasciitis and their temporal changes. We reviewed records of 45 patients treated at our institution between 1979 and 2004. Data about gender, age, etiology, site of involvement, bacteriology, type of...

Author(s): Baris Yildiz, Derya Karakoc, Erhan Hamaloglu, Arif Ozdemir and Ahmet Ozenc

August 2011

Aspects of bacterial colonization in newborn babies

The study of colonization of microbial flora in neonate is very important and finding the causative agent may lead to explore these infections in neonate. In this research the colonization in neonate was measured at the beginning and a few hours after birth. Sampling was performed in operating room, rooming in and nursery section. Some of the neonates deliver by rapture of membrane in normal way and the other deliver by...

Author(s): Fariba Heshmati, Seyeed Amir Yazdanparast , Seyeed Akbar Moosavi , Hussein Dargahi, and Farnaz Tabibzadeh

August 2011

Evaluation of anticarcinogenicity effect of Artemia urmiana by Salmonella typhimurium TA 100 strain

Free radicals are produced during oxidative metabolism in body and they can attack all components of cells and cause cellular changes and early aging. Antioxidant materials role for balance maintenance in cells has currently attracted more attention. The aim of this study is investing of antimutation effect of Artemia, cyst and decapsulated egg extract by Ames test and Salmonella typhimurium TA 100...

Author(s): Masumeh Abbasi, Saman Mahdavi and Sedigheh Mehrabian

August 2011

mRNA expression of iron metabolism relation genes in macrophages by infection with Salmonella typhimurium

A total number of 13 iron metabolism relation genes expression profiles of RAW264.7 murine macrophages infected or uninfected with Salmonella typhimurium were tested by real-time polymerase chain reaction (RT-PCR) to evaluate the metabolism of iron in host-pathogen interplay. The living wild-type S. typhimurium induces expression of the transferrin receptor (Tfr1) in host cell macrophages, which...

Author(s): Pan Xin

August 2011

Expanding drug resistance through integron acquisition in Salmonella spp. isolates obtained in Iran

A total eighty four epidemiologically unrelated clinical isolates of Salmonella entericaserovars were subjected to antimicrobial susceptibility testing and molecular detection of class 1 and 2 integrons. Eleven isolates (13.1%) which were resistant to at least 4 groups of antimicrobial agents considered as MDR (multidrug resistant) Salmonella serovars. PCR assays...

Author(s): Bahareh Rajaei, Seyed Davar Siadat, Mohamad Reza Razavi, Mohammad Reza Aghasadeghi, Nahid Sepehri Rad, Farzad Badmasti, Somieh Khanjani Jafroodi, Taraneh Rajaei, Arfa Moshiri, Saifuddin Javadian

August 2011

The synthesis and role of hydroxyectoine in halophilic bacterium Halomonas ventosae DL7

Hydroxyectoine and ectoine were accumulated in the moderately halophilic bacteriumHalomonas ventosae DL7 as dominant compatible solutes in response to the external thermo- and osmo-stresses (30-42°C, 1-3 M NaCl). As the byproduct of ectoine, hydroxyectoine was directly converted from ectoine mediated by the ectoine hydroxylase in DL7, and the hydroxylase gene sequence was obtained by PCR. Further, the activity...

Author(s): Daochen Zhu, Chenxiang Wang, Shoko Hosoi-Tanabe, Weimin Zhang and Shinichi Nagata

August 2011

Virulence and characteristics of a new nucleopolyhedrovirus strain of Dendrolimus kikuchii (Lepidoptera: Lasiocampidae)

In present study, a nucleopolyhedrovirus was isolated from the infected larvae ofDendrolimus kikuchii, which is a serious pest for a variety of conifers in China. Ultrastructural observation revealed that this virus is a multiple-nucleocapsid nucleopolyhedrovirus (MNPV), which was encapsulated within the envelope of each virion. Based on the morphological characteristics, it was identified as a new virus and named as...

Author(s): Miao Miao Yang,, Meng Lou Li, Yu Zhu Wang, Liang Jian Qu, Ke Yue Huai, Xue Wen Nie, Lu Qin Qiao, Jin Ying Ding and Yong An Zhang

August 2011

Characterization of Staphylococcus spp strains isolated from hospital, community and environmental in Puebla city, Mexico

We investigated the prevalence of methicillin-resistant staphylococci (MRS) and evaluated the antimicrobial resistance patterns of 284 Staphylococcus strains isolated from two hospitals, as well as from community and the environment in Puebla City, Mexico. Isolates were identified by Gram-stain and biochemical reactions and antimicrobial susceptibility testing was performed by Kirby-Bauer and in some cases by...

Author(s): Ana Marta de los Ángeles Lobo-Sánchez, Patricia Lozano-Zaraín, Ygnacio Martínez-Laguna, Zita Gutiérrez-Cázarez, Antonio Rivera-Tapia, Carmen Torres and Rosa del Carmen Rocha-Gracia,

August 2011

Interaction of Severe acute respiratory syndrome (SARS) nucleocapsid protein with Macrophage migration inhibitory factor protein (MIF)

The nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) is a major structural component of virions, which appears to be a multifunctional protein involved in viral RNA replication and translation. However, how N protein interacts with host protein remains largely elusive. To identify cellular proteins that interact with the N protein and to elucidate the possible involvement of N protein in SARS-Cov...

Author(s): Yang Lin,#, Baohua Wang#, Yijun Liu, Zhigang Yu, Manhua Cui and Haichun Ma

August 2011

Isolation and characterization of high caffeine-tolerant bacterium strains from the soil of tea garden

Microbial biodegradation is an important and promising decaffeination approach because of its low cost and high security. The efficiency of this approach mainly depends on the characteristics of the strain. Twenty bacterium strains were isolated from soil of tea garden and exhibited high caffeine-tolerance, and these strains could grow on the medium supplemented with 20 g L-1 caffeine as the sole source for...

Author(s): Fang-Yuan Fan, Yan Xu, Yue-Rong Liang, Xin-Qiang Zheng, Devajit Borthakur,and Jian-Liang Lu

August 2011

Biopreservative potential of marine lactobacillus spp

Bacteriocins are of special interest due to their potential value as natural preservative. The present study is a trial for production of such bacteriocin from a marine Lactobacillus sp.Lactic acid bacteria (LBA) was isolated from fish gut (Mugil cephalus) and prawn muscle (Peneaus monodon) samples and their density were found to be 5.2 x 107 and 6.4 x 107CFU/g respectively. Various pathogens were...

Author(s): K. Indira, S. Jayalakshmi, A. Gopalakrishnan and M. Srinivasan

August 2011

Ephedra alata as biologically-based strategy inhibit aflatoxigenic seedborne mold

Investigation has been carried out to evaluate the effect of range plant Ephedra alata as biologically-based strategy inhibit growth characters and  aflatoxin production (in vitro andin vivo) of Aspergillus flavus. The aqueous extract of E. alata had significant inhibitory potential against growth as well as aflatoxin production by aflatoxigenic seedborne mold (Aspergillus...

Author(s): Al-Qarawi, A. A., Abd_Allah, E. F. and Hashem Abeer

August 2011

Primary resistance rates of Mycobacterium tuberculosis complex strains isolated from new tuberculosis cases: A 6-year observation

The aim of the study was to evaluate the automated mycobacteria growth ındicator tube (MGIT) for drug susceptibility testing of Mycobacterium tuberculosis and to determine resistance patterns. We used BACTEC MGIT 960 System to determine the susceptibility of M. tuberculosis complex isolates to major anti tuberculous agents. Patients with single and first positive isolates were enrolled in the study....

Author(s): Servet Kayhan, Alper AkgüneÅŸ, Hikmet Tereci and Ümit Tutar

August 2011

Cytokine genes expression in mice hepatocytes during malaria infection

The aim of this study was to investigate the inflammation genes (IL-1β, IL-6, TNF-α and iNos) responses to Plasmodium chabaudi malaria in the liver of female C57BL/6 hepatocytes via mRNA expression due to infection with P. chabaudi at different time points. Mice were injected intraperitoneally (ip) with 106 P. chabaudi-infected erythrocytes and then scarified at days (0, 1,...

Author(s): Saad Alkahtani, AL-Farraj S. A., Saud A. Alarifi, AL-Eissa Mohammed saad and Al-Dahmash B

August 2011

Phylogenetic analysis of the nematicidal actinobacteria from agricultural soil of China

The purpose of this study was to assess the diversity and biocontrol potential of nematicidal actinobacteria from agricultural soils. Two hundred soil samples were collected from 20 provinces (autonomous regions or municipalities) of China, and 4000 actinobacteria isolates were obtained. Of the 4000 isolates evaluated, 533 (13.3% of total) and 488 (12.2%) respectively showed nematicidal properties to...

Author(s): Xu Chuan Kun, Lou Xiao Jun, Xi Jia Qin, Gu Lei, Duan Chang Qun, Mo Ming He, Zhang Ke Qin, Yang Fa Xiang and Fang Dun Huang

August 2011

A new recommended disinfectant for dental instruments

Enduro hand sanitizer is a new disinfecting agent that has recently been introduced as an effective antibacterial, antifungal and antiviral agent. Considering the importance of infection control in dentistry, this study aimed to evaluate the antimicrobial effects of the foam and its ability to disinfect dental surfaces and instruments. An experimental study was designed to evaluate the microbial load of 50 samples which...

Author(s): Jamileh bigom Taheri, Mahin Bakhshi, Sedigheh Bakhtiari, Bahare Nazemi, Fateme Fallah, Sahand Rezaie, Hamed Mortazavi and Somayyeh Azimi

August 2011

The functional roles of the residue tyrosine at position 26 in staphylococcal enterotoxin C2

Bacterial superantigen Staphylococcus aureus enterotoxin C2 (SEC2) is a very potent activator of T cells. Previous crystal studies on SEC2 showed that residue Tyr26 which is located near the T cell receptor (TCR) binding sites could potentially participate in T cell activating. In SEC1, a superantigen highly homologous to SEC2, the residue Val26 played a critical role in stimulating certain Vβ...

Author(s): Hongbo Wang, Junyi Zhou, Mingkai Xu, Huiwen Zhang and Chenggang Zhang

August 2011

Prevalence study of cytomegalovirus (CMV) infection among foreign manpower in Jeddah Saudi Arabia

Human cytomegalovirus (HCMV) is a species-specific DNA virus of the Herpetoviridae family. Cytomegalovirus (CMV) is more widespread in developing countries and in areas of low socio-economic conditions. It causes high morbidity and mortality. After primary infection CMV is not eradicated but establishes life-long infection in its host. CMV dispersed and become dormant or latent in multiple end organs, and can later be...

Author(s): N. A. Redwan, M. M. M. Ahmed, and M. S. H. AL Awfi

August 2011

Antibacterial and cytotoxic activity of Eremurus persicus (Jaub and Spach) Boiss

Traditional medicine has a key role in health care worldwide. Obtaining scientific information about the efficacy and safety of the plants is one of the researcher's goals. In this research, the flowering aerial parts of Eremurus persicus were collected from Golpayegan (Isfahan, Iran) in May 2010. The extract was tested for its antibacterial activity against 4 Gram-positive bacteria strains...

Author(s): Mojdeh Hakemi Vala, Jinous Asgarpanah, Mohammad Hossein Hedayati, Jeilan Shirali and Fatemeh Bagheri Bejestani

August 2011

Optimization of the production of exopolysaccharides by Bacillus thuringiensis 27 in sand biological soil crusts and its bioflocculant activity

To improve the yield of exopolysaccharides (EPS) by Bacillus thuringiensis 27 from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China and to analyze its bioflocculant activity, orthogonal matrix method was used and this method enabled us to obtain maximum EPS production. By studying the optimal medium condition of beef extract 3 g/l, peptone 10 g/l, maltose 40 g/l, and NaCl 4g/l we observed...

Author(s): Z. R. Wang., J. P. Sheng, X. L. Tian, T. T. Wu, W. Z. Liu and L. Shen

August 2011

A simple and efficient method that uses low concentration fetal bovine serum to culture and purify Schwann cells

To develop a simple and efficient method for the isolation of highly purified Schwann cells (SCs) from the sciatic nerves of newborn rats, cells were initially cultured in 10% FBS for 6 days, and then cultured in 2% FBS for 6 days. And we used the immunocytochemical characterization, flow cytometry, RT-PCR, to observe the condition of SCs. After the 12-day treatment protocol, we obtained a high yield of SCs with an...

Author(s): Han Feng, Qu Wei, Jiang Huajun, Fu Chongyang, Lu Ming, Zhang Weiguo and Lv Decheng

August 2011

First report on Enterobacter sakazakii from Sudanese patients

Enterobacter sakazakii (E. sakazakii) has been identified as emerging opportunistic pathogens that can cause enterocolitis, bacteraemia, meningitis, brain abscess, and urinary tract infection. They have been particularly associated with meningitis in neonates where infant milk formulae have been epidemiologically linked to the disease. This study was carried out during the period of November 2008 to March...

Author(s): Humodi A. Saeed⊃ and Rania M. Musallam⊃

August 2011

In search of enteroviruses in water media in Marrakech

Enteroviruses are among the most common viruses infecting the human intestine; they are very widespread in nature and resistant to external agents. They are eliminated in the faeces and contaminate water and food. These viruses cause various clinical syndromes and constitute a big public health problem. The aim of our study was to search for enteroviruses in the water samples. For this, a total of 225 L of tap water...

Author(s): Amina Hssaine, Jawhar Gharbi, Rafik Harrath, Rajae Harrak, Abderrahman Chait, Mahjoub Aouni and Jamal Hafid

August 2011

Laboratory analysis of a fatal meningococcal case due to serogroup B Neisseria meningitidis belonging to ST- 4821 complex

We describle a suspicious Meningococcal death case, confirmating by the laboratory PCR methods. The infection developed rapidly within only 24 h until the patient died. Analyzing the close contact strain and the DNA template extracted from the serum of this patient, we characterized a serogroup B Neisseria meningitidis as the pathogen of this case, which, in terms of sequence typing, belonged to ST-4821...

Author(s): Zengguo Wang, Tiejun Hou, Zhijun Chen, Jinsong Li, Shouzhi Wu, Xiaoguang Wei, Yahui Sun and Quanli Du

August 2011

Genotyping of Hepatitis C virus (HCV) in infected patients from Saudi Arabia

A molecular study was carried to investigate the prevalence of Hepatitis C virus (HCV) genotypes in HCV infected population of Saudi Arabia. A total of 358 HCV-positive clinical specimens (serum, EDTA plasma) with viral loads above 2,000 IU/ml were collected for genotyping. Genotyping of 358 samples revealed four different genotypes including 1 (1a and 1b), 2a, 3 (3a) and 4. The most prevalent genotype was 4...

Author(s): Mohammed Ali M. Marie

August 2011

Optimization of ultrasound-assisted extraction conditions using orthogonal matrix design to enhance the antimicrobial activity of extracts from Cichorium intybus root

  Plant-derived compounds used as alternatives to chemical preservatives have been extensively researched for use as natural medical ingredients or food preservatives. Conditions for the ultrasound-assisted extraction (UAE) of chicory (Cichorium intybus) root (including type of solvent, impregnation time, number of sonication steps and ultrasonic power) were optimized to determine the best extract...

Author(s):   Quanzhen Wang, Haitao Liu, Jinhong Du, Jian Cui, Guo Chen and Yuyan Liu