July 2011
Isolation and characterization of a psychrophilic bacterium producing cold active lactose hydrolyzing enzyme from soil of Mt. Himalaya in Nepal
Psychrophilic bacteria, which grew on lactose as a carbon source, were isolated from soil of Island Peak area at Mt. Himalaya in Nepal, and identified. 148 bacterial isolates were isolated via X-gal hydrolysis on plate. Among these, three isolates, referred to as KNOUC 401, 402 and 403, displayed good cold-adapted growth and activity for hydrolysis of o-nitrophenyl-β-D-galactopyranoside (ONPG) and...
August 2011
Nigerian phages: The first bacteriophages from Tropical Africa
Fifteen morphological bacteriophage types were observed in enrichment cultures of sewage, surface water and human feces collected in Lagos, Nigeria. All phages were tailed and belonged to the Myoviridae, Siphoviridae or Podoviridae families. Most phages could be assigned to well-known morphotypes. Ten of them corresponded to phages of enterobacteria or pseudomonads. Four morphotypes (fKZ, T4, c,...
August 2011
Evaluation of real-time PCR for Mycobacterium ulcerans in endemic region in Côte d’Ivoire
Buruli ulcer (BU) is caused by a mycobacterium called Mycobacterium ulcerans. The events of BU are the skin lesions. The lack of early diagnosis and treatment cause severe disability. Today the emergence to BU in Africa and particularly in Côte d’Ivoire needs faster diagnosis to control and to prevent the infection by M. ulcerans. The surveillance of BU is difficult, because the transmission...
August 2011
Contamination of cattle carcasses by Escherichia coli shiga like toxin with high antimicrobials resistence
During processing of cattle carcasses, contamination may occurs with the transfer of microbiota of animals feaces to carcasses. This contamination many times may be byEscherichia coli carriers of virulence factor as stx and eae genes being classified as Shiga like toxin. Shiga toxin-producing Escherichia coli (STEC) is recognized wordwide as human pathogen. A survey was performed to...
August 2011
Determination of insecticidal toxicity of three species of entomopathogenic spore-forming bacterial isolates against Tenebrio molitor L. (Coleoptera: Tenebrionidae)
Bioassays were conducted using larvae of mealworms, Tenebrio molitor, to determine lethal concentration for five entomopathogenic strains of spore-forming bacteria. Lethal concentration was determined by feeding T. molitor larvae cabbage discs dipped in whole cell cultures of these five strains of bacteria. The strains of bacteria were isolates ofBacillus cereus, Bacillus...
August 2011
What changed in necrotizing fasciitis in twenty-five years?
Necrotizing fasciitis (NF) is a deadly soft tissue infection characterized by necrosis of subcutaneous tissues. In this study, our aim was to identify variables affecting patient outcome and mortality in necrotizing fasciitis and their temporal changes. We reviewed records of 45 patients treated at our institution between 1979 and 2004. Data about gender, age, etiology, site of involvement, bacteriology, type of...
August 2011
Aspects of bacterial colonization in newborn babies
The study of colonization of microbial flora in neonate is very important and finding the causative agent may lead to explore these infections in neonate. In this research the colonization in neonate was measured at the beginning and a few hours after birth. Sampling was performed in operating room, rooming in and nursery section. Some of the neonates deliver by rapture of membrane in normal way and the other deliver by...
August 2011
Evaluation of anticarcinogenicity effect of Artemia urmiana by Salmonella typhimurium TA 100 strain
Free radicals are produced during oxidative metabolism in body and they can attack all components of cells and cause cellular changes and early aging. Antioxidant materials role for balance maintenance in cells has currently attracted more attention. The aim of this study is investing of antimutation effect of Artemia, cyst and decapsulated egg extract by Ames test and Salmonella typhimurium TA 100...
August 2011
mRNA expression of iron metabolism relation genes in macrophages by infection with Salmonella typhimurium
A total number of 13 iron metabolism relation genes expression profiles of RAW264.7 murine macrophages infected or uninfected with Salmonella typhimurium were tested by real-time polymerase chain reaction (RT-PCR) to evaluate the metabolism of iron in host-pathogen interplay. The living wild-type S. typhimurium induces expression of the transferrin receptor (Tfr1) in host cell macrophages, which...
August 2011
Expanding drug resistance through integron acquisition in Salmonella spp. isolates obtained in Iran
A total eighty four epidemiologically unrelated clinical isolates of Salmonella entericaserovars were subjected to antimicrobial susceptibility testing and molecular detection of class 1 and 2 integrons. Eleven isolates (13.1%) which were resistant to at least 4 groups of antimicrobial agents considered as MDR (multidrug resistant) Salmonella serovars. PCR assays...
August 2011
The synthesis and role of hydroxyectoine in halophilic bacterium Halomonas ventosae DL7
Hydroxyectoine and ectoine were accumulated in the moderately halophilic bacteriumHalomonas ventosae DL7 as dominant compatible solutes in response to the external thermo- and osmo-stresses (30-42°C, 1-3 M NaCl). As the byproduct of ectoine, hydroxyectoine was directly converted from ectoine mediated by the ectoine hydroxylase in DL7, and the hydroxylase gene sequence was obtained by PCR. Further, the activity...
August 2011
Virulence and characteristics of a new nucleopolyhedrovirus strain of Dendrolimus kikuchii (Lepidoptera: Lasiocampidae)
In present study, a nucleopolyhedrovirus was isolated from the infected larvae ofDendrolimus kikuchii, which is a serious pest for a variety of conifers in China. Ultrastructural observation revealed that this virus is a multiple-nucleocapsid nucleopolyhedrovirus (MNPV), which was encapsulated within the envelope of each virion. Based on the morphological characteristics, it was identified as a new virus and named as...
August 2011
Characterization of Staphylococcus spp strains isolated from hospital, community and environmental in Puebla city, Mexico
We investigated the prevalence of methicillin-resistant staphylococci (MRS) and evaluated the antimicrobial resistance patterns of 284 Staphylococcus strains isolated from two hospitals, as well as from community and the environment in Puebla City, Mexico. Isolates were identified by Gram-stain and biochemical reactions and antimicrobial susceptibility testing was performed by Kirby-Bauer and in some cases by...
August 2011
Interaction of Severe acute respiratory syndrome (SARS) nucleocapsid protein with Macrophage migration inhibitory factor protein (MIF)
The nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) is a major structural component of virions, which appears to be a multifunctional protein involved in viral RNA replication and translation. However, how N protein interacts with host protein remains largely elusive. To identify cellular proteins that interact with the N protein and to elucidate the possible involvement of N protein in SARS-Cov...
August 2011
Isolation and characterization of high caffeine-tolerant bacterium strains from the soil of tea garden
Microbial biodegradation is an important and promising decaffeination approach because of its low cost and high security. The efficiency of this approach mainly depends on the characteristics of the strain. Twenty bacterium strains were isolated from soil of tea garden and exhibited high caffeine-tolerance, and these strains could grow on the medium supplemented with 20 g L-1 caffeine as the sole source for...
August 2011
Biopreservative potential of marine lactobacillus spp
Bacteriocins are of special interest due to their potential value as natural preservative. The present study is a trial for production of such bacteriocin from a marine Lactobacillus sp.Lactic acid bacteria (LBA) was isolated from fish gut (Mugil cephalus) and prawn muscle (Peneaus monodon) samples and their density were found to be 5.2 x 107 and 6.4 x 107CFU/g respectively. Various pathogens were...
August 2011
Ephedra alata as biologically-based strategy inhibit aflatoxigenic seedborne mold
Investigation has been carried out to evaluate the effect of range plant Ephedra alata as biologically-based strategy inhibit growth characters and aflatoxin production (in vitro andin vivo) of Aspergillus flavus. The aqueous extract of E. alata had significant inhibitory potential against growth as well as aflatoxin production by aflatoxigenic seedborne mold (Aspergillus...
August 2011
Primary resistance rates of Mycobacterium tuberculosis complex strains isolated from new tuberculosis cases: A 6-year observation
The aim of the study was to evaluate the automated mycobacteria growth ındicator tube (MGIT) for drug susceptibility testing of Mycobacterium tuberculosis and to determine resistance patterns. We used BACTEC MGIT 960 System to determine the susceptibility of M. tuberculosis complex isolates to major anti tuberculous agents. Patients with single and first positive isolates were enrolled in the study....
August 2011
Cytokine genes expression in mice hepatocytes during malaria infection
The aim of this study was to investigate the inflammation genes (IL-1β, IL-6, TNF-α and iNos) responses to Plasmodium chabaudi malaria in the liver of female C57BL/6 hepatocytes via mRNA expression due to infection with P. chabaudi at different time points. Mice were injected intraperitoneally (ip) with 106 P. chabaudi-infected erythrocytes and then scarified at days (0, 1,...
August 2011
Phylogenetic analysis of the nematicidal actinobacteria from agricultural soil of China
The purpose of this study was to assess the diversity and biocontrol potential of nematicidal actinobacteria from agricultural soils. Two hundred soil samples were collected from 20 provinces (autonomous regions or municipalities) of China, and 4000 actinobacteria isolates were obtained. Of the 4000 isolates evaluated, 533 (13.3% of total) and 488 (12.2%) respectively showed nematicidal properties to...
August 2011
A new recommended disinfectant for dental instruments
Enduro hand sanitizer is a new disinfecting agent that has recently been introduced as an effective antibacterial, antifungal and antiviral agent. Considering the importance of infection control in dentistry, this study aimed to evaluate the antimicrobial effects of the foam and its ability to disinfect dental surfaces and instruments. An experimental study was designed to evaluate the microbial load of 50 samples which...
August 2011
The functional roles of the residue tyrosine at position 26 in staphylococcal enterotoxin C2
Bacterial superantigen Staphylococcus aureus enterotoxin C2 (SEC2) is a very potent activator of T cells. Previous crystal studies on SEC2 showed that residue Tyr26 which is located near the T cell receptor (TCR) binding sites could potentially participate in T cell activating. In SEC1, a superantigen highly homologous to SEC2, the residue Val26 played a critical role in stimulating certain Vβ...
August 2011
Prevalence study of cytomegalovirus (CMV) infection among foreign manpower in Jeddah Saudi Arabia
Human cytomegalovirus (HCMV) is a species-specific DNA virus of the Herpetoviridae family. Cytomegalovirus (CMV) is more widespread in developing countries and in areas of low socio-economic conditions. It causes high morbidity and mortality. After primary infection CMV is not eradicated but establishes life-long infection in its host. CMV dispersed and become dormant or latent in multiple end organs, and can later be...
August 2011
Antibacterial and cytotoxic activity of Eremurus persicus (Jaub and Spach) Boiss
Traditional medicine has a key role in health care worldwide. Obtaining scientific information about the efficacy and safety of the plants is one of the researcher's goals. In this research, the flowering aerial parts of Eremurus persicus were collected from Golpayegan (Isfahan, Iran) in May 2010. The extract was tested for its antibacterial activity against 4 Gram-positive bacteria strains...
August 2011
Optimization of the production of exopolysaccharides by Bacillus thuringiensis 27 in sand biological soil crusts and its bioflocculant activity
To improve the yield of exopolysaccharides (EPS) by Bacillus thuringiensis 27 from sand biological soil crusts in Gurban Tonggut Desert, Xinjiang, China and to analyze its bioflocculant activity, orthogonal matrix method was used and this method enabled us to obtain maximum EPS production. By studying the optimal medium condition of beef extract 3 g/l, peptone 10 g/l, maltose 40 g/l, and NaCl 4g/l we observed...
August 2011
A simple and efficient method that uses low concentration fetal bovine serum to culture and purify Schwann cells
To develop a simple and efficient method for the isolation of highly purified Schwann cells (SCs) from the sciatic nerves of newborn rats, cells were initially cultured in 10% FBS for 6 days, and then cultured in 2% FBS for 6 days. And we used the immunocytochemical characterization, flow cytometry, RT-PCR, to observe the condition of SCs. After the 12-day treatment protocol, we obtained a high yield of SCs with an...
August 2011
First report on Enterobacter sakazakii from Sudanese patients
Enterobacter sakazakii (E. sakazakii) has been identified as emerging opportunistic pathogens that can cause enterocolitis, bacteraemia, meningitis, brain abscess, and urinary tract infection. They have been particularly associated with meningitis in neonates where infant milk formulae have been epidemiologically linked to the disease. This study was carried out during the period of November 2008 to March...
August 2011
In search of enteroviruses in water media in Marrakech
Enteroviruses are among the most common viruses infecting the human intestine; they are very widespread in nature and resistant to external agents. They are eliminated in the faeces and contaminate water and food. These viruses cause various clinical syndromes and constitute a big public health problem. The aim of our study was to search for enteroviruses in the water samples. For this, a total of 225 L of tap water...
August 2011
Laboratory analysis of a fatal meningococcal case due to serogroup B Neisseria meningitidis belonging to ST- 4821 complex
We describle a suspicious Meningococcal death case, confirmating by the laboratory PCR methods. The infection developed rapidly within only 24 h until the patient died. Analyzing the close contact strain and the DNA template extracted from the serum of this patient, we characterized a serogroup B Neisseria meningitidis as the pathogen of this case, which, in terms of sequence typing, belonged to ST-4821...
August 2011
Genotyping of Hepatitis C virus (HCV) in infected patients from Saudi Arabia
A molecular study was carried to investigate the prevalence of Hepatitis C virus (HCV) genotypes in HCV infected population of Saudi Arabia. A total of 358 HCV-positive clinical specimens (serum, EDTA plasma) with viral loads above 2,000 IU/ml were collected for genotyping. Genotyping of 358 samples revealed four different genotypes including 1 (1a and 1b), 2a, 3 (3a) and 4. The most prevalent genotype was 4...
August 2011
Optimization of ultrasound-assisted extraction conditions using orthogonal matrix design to enhance the antimicrobial activity of extracts from Cichorium intybus root
Plant-derived compounds used as alternatives to chemical preservatives have been extensively researched for use as natural medical ingredients or food preservatives. Conditions for the ultrasound-assisted extraction (UAE) of chicory (Cichorium intybus) root (including type of solvent, impregnation time, number of sonication steps and ultrasonic power) were optimized to determine the best extract...
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