Thermophilic bacteria produce commercially useful proteases, active at high temperature. Due to growing demand of proteases application in different industries, the present study was designed to isolate protease producing Bacillus strains. Nine strains were isolated from three different ecological sources and qualitatively screened on skim milk agar. TheBacillus strain PB1 showed 5.4 cm zone of hydrolysis. The screened protease producing strain was further identified on the basis of morphological, biochemical (API 20 and API 50 CHB) and 16 s rRNA molecular identification as Bacillus licheniformis PB1 (Accession EU650317).
Key words: Thermophilic bacteria, proteases, 16S rRNA.
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