African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Isolation, characterization and antimicrobial activity of halophilic bacteria in foreshore soils

Aarzoo Irshad1, Irshad Ahmad2* and Seung Bum Kim1
1Department of Microbiology and Molecular Biology, Chungnam National University, 220 Gung-dong, Yuseong, Daejeon 305-764, Korea. 2Chemical Resources Laboratory, Bio-Resources Division, Tokyo Institute of Technology,4259 Nagatsuta-cho, Midori-ku, Yokohama, 226-8503, Tokyo, Japan
Email: [email protected]

  •  Accepted: 08 January 2013
  •  Published: 31 January 2013


Halophilic bacteria are commonly found in natural environments containing significant concentration of NaCl. Two hundred halophilic bacteria were isolated. Among which, 63 strains were tested for the antimicrobial activity against 10 pathogenic bacteria, three yeast and nine pathogenic fungi. Of these isolates, only 12 strains showed activity against the test microorganisms, namely Pseudomonas aeruginosa, Serratia marcescens,Enterobacter cloacaeStaphylococcus aureusSalmonella typhiKlebsiella pneumoniae,Micrococcus luteusEscherichia coliBacillus subtilisCandida albicansColletotrichumgloeosporioidesFusarium solani and Alternaria alternata. These 12 active isolates were identified to be of the genera, Streptomyces (seven strains), Microbacterium (one strain),Micrococcus (one strain), Bacillus (one strain), Planococcus (one strain) andMarinobacter (one strain). Seven strains were active against pathogenic bacteria and five strains against pathogenic fungi. Two strains SC-2 and SC-13 were found to have a broad spectrum of activity against all tested pathogenic bacteria and yeasts but not against pathogenic fungi. The strains SC-2 and SC-13 were closely related to Microbacterium oxydans (99%) and Streptomyces fradiae (99%) respectively. These findings show that the foreshore soil of Daecheon Beach and Saemangeum Sea of Korea represents an untapped source of bacterial biodiversity, and also that most actinobacterial isolates are capable of antibacterial and antifungal metabolite production.


Key words: Culturable diversity, halophilic bacteria, foreshore soil