January 2013
Study of genotypes and subgenotypes of Hepatitis B virus prevalent in big cities of Pakistan
Hepatitis B virus (HBV) is a well known agent of acute and chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. Around 400 million people worldwide carry the virus including 4.5 million only in Pakistan. Very limited research work on HBV genotyping has been done so far in Pakistan and there is a high level of discrepancy and inconsistency among different group of researchers. The aim of present study was to...
January 2013
Single nucleotide polymorphism (SNP) identification and characterization of goat RAB27A gene in different populations
The 11483 bp of goat RAB27A gene from intron 1 to exon 6 with complete CDS (666 bp) encoding 221 amino acids was obtained (EU400605.2), in which 33 SNPs were found, of which 2 in exons (g.183C>T and g.9669C＞A), 5 in UTR (g.147G>A in 5’UTR,g.9967T>C, g.10101G>A, g.10459G>C and g.10483C>A in 3’UTR), and 26 in introns. Three SSRs (ga, tg and...
January 2013
Isolation, characterization and antimicrobial activity of halophilic bacteria in foreshore soils
Halophilic bacteria are commonly found in natural environments containing significant concentration of NaCl. Two hundred halophilic bacteria were isolated. Among which, 63 strains were tested for the antimicrobial activity against 10 pathogenic bacteria, three yeast and nine pathogenic fungi. Of these isolates, only 12 strains showed activity against the test microorganisms, namely Pseudomonas...
January 2013
Evaluation of the antibacterial properties and synergistic effect of Garcinia kola Heckel (Family: Guttiferae) seed extract and honey on some bacteria
The antibacterial activity of aqueous extract of Garcinia kola, honey and mixture of aqueous G. kola extract and honey was investigated against Escherichia coli, Klebsiella pneumonia, Staphylococcus aureus, Bacillus subtilis, Salmonella sp. and Pseudomonas aeruginosa using agar well diffusion method. Sensitivity pattern of these bacteria was varied and extract...
January 2013
Inhibition of foodborne pathogens by nisin-producing Lactococcus lactis WNC20 in two model foods
The inhibitory effect of Lactococcus lactis WNC20 (Ll20), a nisin-producing lactic acid bacteria, on food-borne pathogens was investigated by adding Ll20 at ~7 log CFU mL-1and or one of four different pathogenic bacteria at ~2 to 4 log CFU mL-1 to the onset of yoghurt and Nham ferments. Enumeration and identification of the bacteria in the yoghurt and Nham were then monitored during the complete...
January 2013
Discriminative multiplex (hexaplex) PCR strategy for the detection of methicillin resistance and virulence factors in Staphylococcus aureus
In clinical microbiology, phenotypic characterization is laborious as well as time consuming strategy and remains less discriminative among high virulent to less virulent clinically important strains. Induction of molecular techniques, allow more accurate and quick way out for identification of Staphylococcus aureus along with its virulence capabilities. This study describes a multiplex (hexaplex) strategy for...
January 2013
DNA-binding activity and in vivo cytotoxicity of Ganoderma applanatum (Pers.) Pat. supercritical-CO2 extracts
Ganoderma applanatum (Pers.) Pat. is a perennial and abundant mushroom in Bukidnon Province, Philippines but its bioactivities have just been investigated lately. In this study, three supercritical-CO2 (SC-CO2) extracts (GA1, GA2 and GA3) from the local strain ofG. applanatum (Pers.) Pat. were examined for their DNA-binding potential by means of biomolecular-chemical screening and in...
January 2013
Aerobic denitrifying characteristics of duckweed rhizosphere bacterium RWX31
Aerobic denitrifying characteristics of duckweed rhizosphere bacterium RWX31 was studied in this paper. Bacterium RWX31 exhibited efficient growth stability and nitrogen removal ability, with nitrate removal efficiency of 81.3% at an initial 140 mg l-1 NO3-N. Under aerobic conditions, the maximum nitrate-N removal rates were 30.9, 24.3 and 11.5 mg l-1 h-1, with nitrate, nitrite and ammonium as sole nitrogen...
January 2013
Self-pasteurised substrate for growing oyster mushrooms (Pleurotus spp.)
As part of participatory research with farmers from the municipality of Cacahoatán, Chiapas, Mexico, an experiment was conducted to determine the technical feasibility of using substrate self-pasteurisation to grow oyster mushrooms (Pleurotus spp). In the first step, the substrate treatment (alkaline immersion disinfection versus self-heating pasteurisation) was evaluated, and after determining the better...
January 2013
Optimization of fermentation process for preparation of mulberry fruit wine by response surface methodology
Response surface methodology (RSM) has been the most widely used optimization method in recent years. Lately, many studies successfully employed this methodology to improve enzyme production processes of industrial interest. In this context, the mulberry (Morus alba L.) fruit was used as raw material to brew fruit wine. The effects of fermentation parameters (temperature, pH value, inoculum size and...
January 2013
Attenuated HtrA-mutant of Streptococcus pneumoniae induces protection in murine models of pneumococcal pneumonia and bacteraemia
Streptococcus pneumoniae (the pneumococcus) is a Gram-positive human pathogen that causes a number of diseases such as pneumonia, otitis media, bacteraemia, and meningitis. The increasing rate by which the pneumococcus acquires resistance to the widely used antibiotics and the limitations of currently licensed vaccines have made pneumococcal diseases a major health problem. Search for more effective vaccines is...
January 2013
Effect of overexpression of proline dehydrogenase on high saline adaptation through proline utilization in Escherichia coli
The effect of the amount of proline dehydrogenase PutA expression on the mechanism of adaptation of Escherichia coli cells under high salinity was analyzed. The PutA gene derived from E. coli expression plasmid was introduced into E. coli cell, and PutA high expression strain BL21-PutA was developed. Transcription of the PutA was induced by the addition of gene expression inducer IPTG, and...
January 2013
Polygalacturonase production from Rhizomucor pusillus isolated from fruit markets of Uttar Pradesh
Polygalacturonase (PGases) production from Rhizomucor pusillus was enhanced when various production parameters were optimized. Maximum PGases activity was obtained after 120 h at 45°C and pH 5.0 with pectin (pure) (1.5% w/v) as carbon source, urea (0.3% w/v) as nitrogen source and MnSO4 (0.2% w/v) as mineral supplement. PGases from R.pusillus was partially purified and subjected to characterization...
January 2013
Study on liquid paraffin decomposer microorganisms isolated from soils of Julfa-Iran
Paraffin compounds are derived from petroleum. Natural environment pollution with petroleum or petroleum products is still an environmental problem. The objective of the present study was to isolate the environmental liquid paraffin decomposer strains isolated from soil in Julfa, Iran. Initially, we collected soil samples from different areas and by preparation of serial dilution for 10-1 to 10-6 of them and...
January 2013
Isolation and characterization of phosphate solubilizing bacteria and their co-inoculation efficiency on tomato plant growth and phosphorous uptake
Plant growth promoting traits which include indoleacetic acid (IAA), ammonia, siderophore and hydrogen cyanide (HCN) production were assessed in two phosphate solubilizing bacterial (PSB) isolates (Pantoea agglomerans and Burkholderia anthina) and their effect on growth and phosphorous uptake of tomato plants was investigated with a pot experiment conducted under green house conditions. The pots were arranged...
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