African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Short Communication

Development of a multiplex polymerase chain reaction (PCR) with an internal control method to detect Yersinia pestis in the plague foci surveillance

Zhikai Zhang, Ying Liang, Dongzheng Yu, Lianxu Xia* and Rong Hai*  
State Key Laboratory for Infectious Disease Prevention and Control, Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Email: [email protected], [email protected]

  •  Accepted: 07 February 2013
  •  Published: 19 February 2013

Abstract

To detect Yersinia pestis in the foci surveillance rapidly and specially, we developed a multiplex polymerase chain reaction (PCR) with an internal control technique. The specific segments of Yesinia pestis were co-amplified by PCR directly from the hosts’ organ samples with an internal control to prevent false negative results that might be caused by PCR inhibitors. Comparing the PCR results with that of the bacteria culture, the positive detection rate of PCR method was higher and it was more rapid. So, it is a useful method for the detection of Yesinia pestis in the plague foci surveillance.

 

Key words: multiplex polymerase chain reaction (PCR), internal control,Yersinia pestis.