February 2013
Isotope effects on cell growth and sporulation, and spore heat resistance, survival and spontaneous mutation of Bacillus cereus by deuterium oxide culture
Deuterated spores of Bacillus cereus were prepared using a multi-stage adaptation protocol and then preserved at -20, 4, 25 and 40°C, respectively, to investigate the genetic mutation effects of deuterium oxide (D2O) isotope. The effects of the D2O isotope on cell growth and sporulation, and spore heat resistance, survival and spontaneous mutation rate of spores were examined. The results suggested...
February 2013
RT-PCR assays for the evaluation of the expression of Listeria monocytogenes virulence genes after cold and freezing shock
Listeria monocytogenes is a food-borne bacterial pathogen that is able to grow at refrigeration temperatures and resist freezing. This organism frequently colonizes and proliferates on preserved food products despite exposure to stress conditions induced by low storage temperatures. To investigate microbial virulence genes expression associated with cold shock, RT-PCR (Reverse Transcript PCR) assays was...
February 2013
Rapid detection of emetic toxin producing Bacillus cereus strains using triple-primer polymerase chain reaction (PCR) assay
Emetic toxin producing Bacillus cereus can cause emetic food poisoning. In this study, an improved triple-primer polymerase chain reaction (PCR) assay was developed as a reliable and rapid identification method for the detection of emetic toxin producing B. cereus strains based on the unique gene sequences of the CER, ces and groEL. Specificity and sensitivity of the primers was...
February 2013
Solubilization of inorganic phosphate and production of organic acids by bacteria isolated from a Moroccan mineral phosphate deposit
Three efficient inorganic-phosphate solubilizing bacteria (PSB) were isolated from a phosphate rock deposit of a Moroccan mine. The phosphate solubilization index of these isolates, determined in National Botanical Research Institute's phosphate (NBRIP) medium supplemented with tribasic calcium phosphate, ranging from 2.8 to 4.4. The medium pH dropped from 7.0 to 3.5 units after growth under continuous agitation for...
February 2013
An innovative microwave process for microbial decontamination of spices and herbs
The microbiological status of some dried spices and herbs from local markets in Jordanand the effect of home microwave on reducing their microbial load were evaluated. The results indicated that the sixteen tested samples were contaminated with aerobic mesophilic bacteria ranging from 1.18 x 103 to 5.1 x 105 CFU/g, mesophilic spore former bacteria 1.2 x 102 to 1.2 x 104 CFU/g,...
February 2013
The detection and characterization of multiple tick-borne pathogens in cattle at Ficksburg and Reitz (Free State Province, South Africa) using reverse line blot hybridization
Ticks and tick-borne diseases are widely distributed in southern Africa and limit livestock production of commercial farmers and the subsistence economy of the resource-poor farmers. Reverse line blot hybridization was used to survey the prevalence of tick-borne pathogens in cattle of commercial and small scale resource-poor farmers within the north-eastern region of the Free State Province, South Africa. Blood was...
February 2013
Antagonistic mechanism and control effect of Bacillus subtilis Y2 against Fusarium oxysporum causing soybean root rot
Soybean root rot caused by Fusarium oxysporum is a destructive soilborne pathogen and is of economic importance in all soybean producing areas. Bacillus subtilis strain Y2 is a potential bacterial biocontrol agent against F. oxysporum. An antagonistic mechanism ofB. subtilis Y2 against F. oxysporum was conducted. The antagonistic mechanism of strain Y2 against F....
February 2013
Molecular characterization and phylogenetic study of Newcastle disease virus isolates from the 2010 to 2011 outbreaks in Shiraz, Iran
The velogenic Newcastle disease virus (NDV) causes a devastating form of disease in commercial poultry. In this study, we characterized 10 isolates of NDVs that were obtained from recent outbreaks in commercial broiler chickens in Shiraz (in the Southwest of Iran) during 2010 and 2011. The F protein gene was amplified by RT-PCR using kit with F gene specific primers. A piece of 1561 nucleotide including F gene cleavage...
February 2013
Antibacterial effect of hydrosoluble extracts of garlic (Allium sativum) against Bifidobacterium spp. and Lactobacillus acidophilus
The antimicrobial effects of garlic (Allium sativum) against pathogenic microorganisms have been well documented. It is generally stated that garlic exhibits differential inhibition between pathogenic and beneficial bacteria. Though there is substantial evidence to support the claim for pathogens, there is limited literature on its effects on beneficial bacteria, specifically probiotic bifidobacteria. This study...
February 2013
Genetic characterization of VP6 and NS4 genes of bluetongue viruses isolated in Israel during 2006 to 2011
Twenty-nine (29) Israeli bluetongue virus isolates belonging to eight different serotypes (BTV-2, 4, 5, 8, 12, 15, 16, and 24) were selected to study the nucleotide sequences of segment 9 encoding VP6 protein and the non-structural protein, NS4. Phylogenetic analysis revealed that all Israeli BTV isolates were subdivided into three main groups, of which the 1st and 2nd groups each...
February 2013
Antibiotic susceptibility testing of isolated Bacillus thuringiensis from three soil types around Iligan City, Philippines
This study was conducted with the aim of isolating and identifying entomopathogenic isolate of Bacillus thuringiensis (Bt) from the three different kinds of soils and to determine its antibiotic susceptibility. Soil samples were taken from three different uncultivated sites that have no history of treatment with Bt products. Isolation of the Bt strain was conducted based on the conventional...
February 2013
Isolation and functional study of potentially probiotic Lactobacilli from Taiwan traditional paocai
The objective of this study was to isolate, characterize and identify lactic acid bacteria (LAB) from Taiwan traditional pickled paocai. A total of 207 acid-producing strains were isolated and identified as catalase negative, Gram positive, with rod or bead shape and immobility by microbial and biochemical tests. None of the assayed strains showed haemolytic and gelatinase...
February 2013
CTX-M-15 type extended spectrum β-lactamase producing Escherichia coli and Klebsiella pneumoniae: A developing problem in infected outpatients and hospitalised patients in Istanbul, Turkey
Extented-spectrum β-lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae were shown to be a significant cause of both community-acquired and hospital-acquired infections worldwide. The aim of this study was to compare the epidemiological feature of CTX-M, TEM and SHV producing pathogenic E. coli and K. pneumoniae strains in outpatients and hospitalised...
February 2013
Development of a multiplex polymerase chain reaction (PCR) with an internal control method to detect Yersinia pestis in the plague foci surveillance
To detect Yersinia pestis in the foci surveillance rapidly and specially, we developed a multiplex polymerase chain reaction (PCR) with an internal control technique. The specific segments of Yesinia pestis were co-amplified by PCR directly from the hosts’ organ samples with an internal control to prevent false negative results that might be caused by PCR inhibitors. Comparing the PCR results...
February 2013
Flagellin complete sequence as an inter-specific molecular phylogenetic marker among bacteria
Flagellin, a flagelar subunit protein, presents four structural domains, two of which are highly conserved among bacteria, while the other two tend to be variable. Flagellins have been used as phylogenetic molecular markers, mainly intra-specifically. In this work, a marker comparison for bacterial phylogenetic reconstruction was performed by means of five different DNA sequences: the conserved and complete sequence of...
Advertisement
Advertisement
