African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

The quantity and quality of methanogenic microorganisms based on rRNA analysis and their relation to methane production

Maciej Walczak and Maria Swiontek Brzezinska*
Department of Environmental Microbiology and Biotechnology, Nicolaus Copernicus University, Gagarina 9, 87-100 Torun, Poland.
Email: [email protected]

  •  Accepted: 12 June 2013
  •  Published: 25 June 2013


Biogas plants continuously convert biological wastes mainly into a mixture of methane, CO2 and H2O a conversion that is carried out by a consortium of bacteria and archaea. The microbial community structure of the fermentation process was evaluated by using oligonucleotide probes complementary to conserved sequences of the 16S rRNAs of phylogenetically defined groups of methanogens. At the beginning of fermentation, Eubacteria are the main group and the count of Archaea then constitutes only an insignificant percentage of the population of microorganisms. During the process of fermentation, we recorded a very significant increase in methanogenic microorganisms after 40 days of the process in progress. It is reflected in the quantity and the composition of released biogas.


Key words: Methane fermentation, methanogenic microorganisms, phylogenetic diversity, rRNA.