African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Antimicrobial potential and chemical constituent of Mikania micrantha H. B. K.

Yan Li1, Bin-bin Shen2, Jun Li1,2, Yuan Li1, Xia-xia Wang3, and Ao-cheng Cao1*
1Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P. R. China. 2College of Resources and Environment, South China Agricultural University, Guangzhou, Guangdong, 510642, P. R. China. 3Anhui Academy of Agricultural Sciences, Hefei 230031, P. R. China.
Email: [email protected]

  •  Accepted: 26 April 2013
  •  Published: 14 May 2013


The aerial parts of Mikania micrantha H. B. K. were prepared with different polarities solvents by the associated ultrasonic extracting method. The screening antimicrobial activities assays were designed against fungi (Fusarium solani, Phytophthora parasiticaDestur, Pythium aphanidermatum, and Rhizoctonia solani Kühn) and bacteria (Ralstonia dolaanacearum, Xanthomonas oryzae pv. Oryzae). The results show that the leaves extracts were higher bioactivity than the stem extracts, and the chloroform extracts were observed the highest bioactivity. The antimicrobial potential of the leaves extracts of M. micrantha were also assessed. The results show that concentration for 50% of maximal effect (EC50) values was 1313.92, 2493.63, 1718.97, and 1084.91 mg/l respectively at 48h against the five fungi testedDetermination of Minimum Inhibitory Concentration (MIC) values were found to be 250 and 500 mg/l respectively against the two bacteria tested. The result of Gas Chromatography-Mass Spectrometer (GC-MS) analysis indicated that 13 compounds, which represented 87.03% of total, were present in the leaves extract, and 1-(4-chlorophenyl)-2- hydroxyethanone (57.44%) was the major chemical constituent.


Key words: Mikania micrantha H.B.K., leaves extracts, chloroform extracts, fungistasis efficacybacteriostatic efficacyGC-MS analysis