African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Optimal conditions for maximizing production of reducing sugars from microwave-assisted FeCl3 pretreated rice straw degraded by Trichoderma viride and Bacillus pumilus

Ji-Liang Lü and Pei-Jiang Zhou*
School of Resource and Environmental Science, Hubei Biomass-Resource Chemistry and Environmental Biotechnology Key Laboratory, Wuhan University, Wuhan 430079, China.
Email: [email protected]

  •  Accepted: 21 October 2011
  •  Published: 23 December 2011



Microwave-assisted FeCl3 pretreated rice straw was degraded to reducing sugars withTrichoderma viride and Bacillus pumilus. Response surface methodology (RSM) and Box-Behnken design (BBD) were used to optimize degradation conditions of pretreated rice straw with respect to initial pH, degradation temperature, T. viride/B. pumilusinoculum ratio (T/B inoculum ratio) and degradation time to achieve the highest production of reducing sugars. The maximum production of reducing sugars obtained was 7.19 g/L at the optimal conditions of initial pH, 8; degradation temperature, 32°C; T/B inoculum ratio, 3 ml/1 ml; degradation time, 60 h. This optimization strategy enhanced the production of reducing sugars from 6.62 to 7.19 g/L after optimization, which shows the beneficial effect on optimization of degradation conditions.


Key words: Microwave-assisted FeCl3 pretreated rice straw, Trichoderma virideBacillus pumilus, degradation conditions, response surface methodology (RSM) optimization.