Abstract

We established a new screening method for oleaginous fungus, which was consistent with sudan black B stained but more convenient and faster. 11 strains of oleaginous fungi that utilize D-xylose were isolated from the soil of Qinghai Plateau by this method. These strains belong to 7 species by molecular identifying, they are Tilletiopsis albescens,Backusella ctenidia, lectosphaerella sp., Gibberella fujikuroi, Fusarium sp. Aspergillus fumigatus and Penicillium decumberns; 4 of which were the first time found to produce lipids, they are Tilletiopsis albescens, Backusella ctenidia, lectosphaerella sp. andGibberella fujikuroi. In addition, these oleaginous fungi could accumulate certain microbial oil by fermenting D-xylose and carboxymethyl cellulose (CMC), whose composition is similar to vegetable oil and can be converted to biodiesel. These microorganism species that may use cheap lignocellulose to produce microbial oil, it can pave lane for biodiesel production with low cost.

Key words: Oleaginous fungi, D-xylose, carboxymethyl cellulose, biodiesel.