African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5210

Full Length Research Paper

Isolation, identification and characterization of oleaginous fungi from the soil of Qinghai Plateau that utilize D-xylose

Shi Lin Li, Shun Li Feng, Zeng Ting Li, Hui Xu, Ying Peng Yu, Dai Rong Qiao and Yi Cao*
Microbiology and Metabolic Engineering Key Laboratory of Sichuan Province, College of Life Science, Sichuan University, Chengdu, P. R. China.
Email: [email protected]

  •  Published: 04 August 2011


We established a new screening method for oleaginous fungus, which was consistent with sudan black B stained but more convenient and faster. 11 strains of oleaginous fungi that utilize D-xylose were isolated from the soil of Qinghai Plateau by this method. These strains belong to 7 species by molecular identifying, they are Tilletiopsis albescens,Backusella ctenidialectosphaerella sp., Gibberella fujikuroi, Fusarium sp. Aspergillus fumigatus and Penicillium decumberns; 4 of which were the first time found to produce lipids, they are Tilletiopsis albescensBackusella ctenidialectosphaerella sp. andGibberella fujikuroi. In addition, these oleaginous fungi could accumulate certain microbial oil by fermenting D-xylose and carboxymethyl cellulose (CMC), whose composition is similar to vegetable oil and can be converted to biodiesel. These microorganism species that may use cheap lignocellulose to produce microbial oil, it can pave lane for biodiesel production with low cost.


Key words: Oleaginous fungi, D-xylose, carboxymethyl cellulose, biodiesel.