African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Effects of inoculation with plant growth promoting rhizobacteria (PGPRs) on different growth parameters of cold area rice variety, Fakre malakand

Bashir Ahmed1*, Midrarullah1,2 and M. Sajjad Mirza2
1Pharma biotech Research Laboratory, Centre of Biotechnology and Microbiology, University of Peshawar, KPK, Pakistan. 2National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad, Pakistan.
Email: [email protected]

  •  Accepted: 29 March 2013
  •  Published: 23 April 2013


The present study was conducted to isolate and characterize plant growth promoting rhizobacteria (PGPR) from the rhizosphere of rice grown in cold climate at Swat and to assess their impact on plant growth when used as inoculants. A total of 18 bacterial strains were isolated from roots and rhizosphere of rice. With the exception of 3 strains, all isolates showed IAA production in pure culture. Three bacterial strains (Azospirillum brasilense R1, Azospirillum lipoferum RSWT1 and Pseudomonas Ky1) were used to inoculate rice at two experimental sites in Swat (Agriculture Research Institute (N) Mingora and Udigram). Plant growth promotion was observed in all inoculated treatments over non-inoculated control which was evident from increase in number of tillers, straw and grain yields and total weight of the plants. A. brasilense R1 was more effective in plant growth promotion than other strains. At ARIN Mingora, Swat, inoculation of rice variety Fakre Malakand with A. brasilense R1 increased the straw weight by 16.6%, grain weight by 22.7% over control. Inoculation with A. lipoferum RSWT1 and Pseudomonas Ky1 increased grain weight by 4.8 to 13.5% and 17.3 to 18.5%, respectively. At Udigram, Swat, inoculation with A. brasilense R1 increased the straw weight by 14.2% and grain weight by 22% than the control.


Key words: AzospirillumPseudomonas, inoculation of rice, effects of inoculation.