African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5232

Full Length Research Paper

Resistance to β-lactams of human and veterinary Salmonella isolates in Egypt and Algeria

Abdelhakim Aouf, Yamina Messai, Mohammed S. Salama, Hala M. Aboushady, Mervat G. El-Anany, Souhila Alouache and Rabah Bakour
  1Laboratory of Cellular and Molecular Biology, Faculty of Biological Sciences, University of Science and Technology Houari Boumediene, Algiers, Algeria. 2Microbiology Laboratory, Faculty of Science, Ain Shams University, Cairo, Egypt. 3Molecular Biology Laboratory, Faculty of Science, Ain Shams University, Cairo, Egypt.  4Microbiology Laboratory, Kasr Alainy Hospital, Cairo, Egypt.
Email: [email protected]

  •  Accepted: 28 March 2011
  •  Published: 30 April 2011

Abstract

Seventy six non-typhoid Salmonella were isolated from both human and poultry in Egypt and Algeria and tested for their antibiotics resistance. The incidence of multiple antibiotics resistance was high. To study β-lactams resistance mechanisms, double disk synergy test (DDST) with and without cloxacilline was used, results revealed the production of extended spectrum β-lactamases (ESBLs) and cephalosporinase in seven and one human Egyptian isolates, respectively. The seven ESBL isolates were identified asSalmonella enterica serotype Poona and their molecular typing by ERIC-PCR revealed unrelated genetic patterns, indicating that these isolates are not clonal. The Cephalosporinase-ESBL- producing isolate was identified as S. enterica serotype Hadar. Polymerase chain reaction (PCR) with specific primers showed the presence of blaTEMand blaSHV genes, respectively, in all and four ESBL producers, and blaAmpC gene was detected in cephalosporinase-producing isolate. Genetic transfer by conjugation and plasmid profiles analysis showed that these genes and their resistance markers were transferable in association with plasmids of 60 kb for ESBLs and 64 and 3.2 kb for AmpC cephalosporinase.

 

Key words: non-typhoid Salmonella, extended spectrum β-lactamases, cephalosporinase, Egypt, Algeria