African Journal of
Microbiology Research

  • Abbreviation: Afr. J. Microbiol. Res.
  • Language: English
  • ISSN: 1996-0808
  • DOI: 10.5897/AJMR
  • Start Year: 2007
  • Published Articles: 5233

Full Length Research Paper

Utilization of sugar beet pulp as a substrate for the fungal production of cellulase and bioethanol

  Marzieh Moosavi-Nasab* and Marjan Majdi-Nasab  
  Department of Food Science and Technology, College of Agriculture, Shiraz University, Shiraz, I. R. Iran.  
Email: [email protected], [email protected]

  •  Accepted: 02 November 2010
  •  Published: 04 December 2010

Abstract

 

Microbial production of cellulase and bioethanol were studied using Sugar Beet Pulp (SBP) as a substrate by Trichoderma reesei and Saccharomyces cerevisiae, respectively. The subculture medium for cellulase production was a salt solution. Fungal cells were sub-cultured in an orbital shaker at 30°C for 4 to 6 days and then were used as an inoculum. Exponential cells were inoculated into a medium containing SBP. A maximum cellulase activity of 0.46 IU/ml of filter paper activity was obtained. Ethanol yield was higher in pretreated SBP than in non-treated SBP. After 24 h fermentation period, 0.11 g/L ethanol was produced from pretreated SBP, while non-treated SBP produced 0.05 g/L ethanol.

 

Key words: Trichoderma reesei, Saccharomyces cerevisiae, sugar beet pulp, cellulase,bioethanol.

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This article is published under the terms of the Creative Commons Attribution License 4.0

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