Due to the production of abundant capsular polysaccharides and or exopolysaccharides, some bacteria form mucoid and opaque colonies on solid agar medium. Polysaccharides interfere with the isolation and characterization of DNA. Extraction of polysaccharide-free DNA from the mucoid bacteria is required for genetic and biochemical studies. In this study, mucoid Vibrio parahaemolyticus and Klebsiella pneumonia were used as model organisms for comparing the effectiveness of two DNA extraction methods. The two methods were proved reproducible and cost-effective for extracting high-purity genome DNA from mucoid bacteria for further applications.
Key words: Mucoid bacteria, Vibrio parahaemolyticus, Klebsiella pneumonia, DNA isolation, polysaccharides.
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