Abstract

Toxigenic types of Clostridium perfringens are significant causative agents of enteric disease in domestic animals, although type E is presumably rare, appearing as an uncommon cause of enterotoxemia of lambs, calves and rabbits. In 100 feces samples collected from ostrich farm, 40 strains of C. Perfringens were isolated by biochemical test, of these, 4 strains were toxigenic strains type A and any toxigenic strains of type B and type C and type D are not fond. Enzyme-linked immunosorbent assay (ELISA) kit for detection of type E not exist and isolation of type E not purpose. The technique of isolation and ELISA test are described in this paper.

Key words: Toxigenic strains, Clostridium perfringens, Enzyme-linked immunosorbent assay (ELISA).